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DNA Biosensor for Rapid Detection of Genotoxic Compounds in Soil Samples
Department of Chemistry, University of Florence, Via della Lastruccia 3, 50019 Sesto Fiorentino (Firenze), Italy.
Department of Chemistry, National University of Littoral, University Campus, 3000 Santa Fe, Argentina
Department Industrial Installations and Interaction with the Environment (DIPIA), National Authority for Health and Safety at Work (ISPESL), Via Urbana 167, 00184 Rome, Italy
National Institute of Soil Defence (ISSDS), Via Casette 1, 02100 Rieti, Italy
* Author to whom correspondence should be addressed.
Received: 25 February 2005; Accepted: 30 March 2005 / Published: 14 November 2005
Abstract: An electrochemical DNA-based biosensor is proposed as a fast and easyscreening method for the detection of genotoxic compounds in soil samples. The biosensorwas assembled by immobilising double stranded Calf thymus DNA on screen-printedelectrodes. The interactions between DNA and environmental pollutants can causevariations of the electrochemical proprieties of DNA when they cause a DNA damage.Preliminary studies were performed using benzene, naphthalene and anthracene derivativesas model compounds. The effect of these compounds on the surface-confined DNA wasfound to be linearly related to their concentration in solution. On the other hand, theobjective was to optimise the ultrasonic extraction conditions of these compounds fromartificially spiked soil samples. Then, the applicability of such a biosensor was evaluated byanalysing soil samples from an Italian region with ecological risk (ACNA of Cengio, SV).DNA biosensor for qualitative analysis of soil presented a good correlation with a semiquantitativemethod for aromatic ring systems determination as fixed wavelengthfluorescence and interestingly, according results were found also with other bioassays.This kind of biosensors represent a new, easy and fast way of analysis of polluted sites, therefore they can be used as early warnings devices in areas with ecological risk as in situ measurement.
Keywords: DNA damage; electrochemical DNA biosensors; genotoxicity; soil contamination
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Bagni, G.; Hernandez, S.; Mascini, M.; Sturchio, E.; Boccia, P.; Marconi, S. DNA Biosensor for Rapid Detection of Genotoxic Compounds in Soil Samples. Sensors 2005, 5, 394-410.
Bagni G, Hernandez S, Mascini M, Sturchio E, Boccia P, Marconi S. DNA Biosensor for Rapid Detection of Genotoxic Compounds in Soil Samples. Sensors. 2005; 5(6):394-410.
Bagni, Graziana; Hernandez, Silvia; Mascini, Marco; Sturchio, Elena; Boccia, Priscilla; Marconi, Simona. 2005. "DNA Biosensor for Rapid Detection of Genotoxic Compounds in Soil Samples." Sensors 5, no. 6: 394-410.