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Sensors 2005, 5(6), 411-423; doi:10.3390/s5060411
Article

Voltammetric Detection of Damage to DNA by Arsenic Compounds at a DNA Biosensor

1,* , 1, 1, 1, 2 and 2
Received: 23 February 2005; Accepted: 16 March 2005 / Published: 14 November 2005
(This article belongs to the Special Issue DNA Based Sensors)
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Abstract: DNA biosensor can serve as a powerfull tool for simple in vitro tests of chemicaltoxicity. In this paper, damage to DNA attached to the surface of screen-printed carbonelectrode by arsenic compounds in solution is described. Using the Co(III) complex with1,10-phenanthroline, [Co(phen)3]3+ , as an electrochemical DNA marker and the Ru(II)complex with bipyridyne, [Ru(bipy)3]2+ , as a DNA oxidation catalyst, the portion of originaldsDNA which survives an incubation of the biosensor in the cleavage medium was evaluated.The model cleavage mixture was composed of an arsenic compound at 10-3 mol/Lconcentration corresponding to real contaminated water, 2x10-4 mol/L Fe(II) or Cu(II) ions asthe redox catalyst, and 1.5x10-2 mol/L hydrogen peroxide. DNA damage by arsenite,dimethylarsinic acid as the metabolic product of inorganic arsenic and widely used herbicide,as well as phenylarsonic acid and p-arsanilic acid as the representatives of feed additives wasfound in difference to arsenate.
Keywords: Arsenic; DNA damage; DNA biosensor; Screen-printed electrode; Voltammetry Arsenic; DNA damage; DNA biosensor; Screen-printed electrode; Voltammetry
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Labuda, J.; K. Bubnicova, K.; Kovalova, L.; Vanickova, M.; Mattusch, J.; Wennrich, R. Voltammetric Detection of Damage to DNA by Arsenic Compounds at a DNA Biosensor. Sensors 2005, 5, 411-423.

AMA Style

Labuda J, K. Bubnicova K, Kovalova L, Vanickova M, Mattusch J, Wennrich R. Voltammetric Detection of Damage to DNA by Arsenic Compounds at a DNA Biosensor. Sensors. 2005; 5(6):411-423.

Chicago/Turabian Style

Labuda, J.; K. Bubnicova, K.; Kovalova, L.; Vanickova, M.; Mattusch, J.; Wennrich, R. 2005. "Voltammetric Detection of Damage to DNA by Arsenic Compounds at a DNA Biosensor." Sensors 5, no. 6: 411-423.


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