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Voltammetric Detection of Damage to DNA by Arsenic Compounds at a DNA Biosensor
Department of Analytical Chemistry, Faculty of Chemical and Food Technology, Slovak University of Technology, 81237 Bratislava, Slovakia
UFZ Center for Environmental Research Leipzig-Halle, D-04318 Leipzig, Germany
* Author to whom correspondence should be addressed.
Received: 23 February 2005; Accepted: 16 March 2005 / Published: 14 November 2005
Abstract: DNA biosensor can serve as a powerfull tool for simple in vitro tests of chemicaltoxicity. In this paper, damage to DNA attached to the surface of screen-printed carbonelectrode by arsenic compounds in solution is described. Using the Co(III) complex with1,10-phenanthroline, [Co(phen)3]3+ , as an electrochemical DNA marker and the Ru(II)complex with bipyridyne, [Ru(bipy)3]2+ , as a DNA oxidation catalyst, the portion of originaldsDNA which survives an incubation of the biosensor in the cleavage medium was evaluated.The model cleavage mixture was composed of an arsenic compound at 10-3 mol/Lconcentration corresponding to real contaminated water, 2x10-4 mol/L Fe(II) or Cu(II) ions asthe redox catalyst, and 1.5x10-2 mol/L hydrogen peroxide. DNA damage by arsenite,dimethylarsinic acid as the metabolic product of inorganic arsenic and widely used herbicide,as well as phenylarsonic acid and p-arsanilic acid as the representatives of feed additives wasfound in difference to arsenate.
Keywords: Arsenic; DNA damage; DNA biosensor; Screen-printed electrode; Voltammetry
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MDPI and ACS Style
Labuda, J.; K. Bubnicova, K.; Kovalova, L.; Vanickova, M.; Mattusch, J.; Wennrich, R. Voltammetric Detection of Damage to DNA by Arsenic Compounds at a DNA Biosensor. Sensors 2005, 5, 411-423.
Labuda J, K. Bubnicova K, Kovalova L, Vanickova M, Mattusch J, Wennrich R. Voltammetric Detection of Damage to DNA by Arsenic Compounds at a DNA Biosensor. Sensors. 2005; 5(6):411-423.
Labuda, J.; K. Bubnicova, K.; Kovalova, L.; Vanickova, M.; Mattusch, J.; Wennrich, R. 2005. "Voltammetric Detection of Damage to DNA by Arsenic Compounds at a DNA Biosensor." Sensors 5, no. 6: 411-423.