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Sensors 2005, 5(6), 364-376; doi:10.3390/s5060364
Article

Amperometric DNA-Peroxidase Sensor for the Detection of Pharmaceutical Preparations

* , , ,  and
Analytical Chemistry Department, Chemistry Faculty, Kazan State Uni 08 Kazan, Russia
* Author to whom correspondence should be addressed.
Received: 2 February 2005 / Accepted: 9 March 2005 / Published: 14 November 2005
(This article belongs to the Special Issue DNA Based Sensors)
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Abstract

Novel DNA-sensor with enzymatic amplification of the signal has beendeveloped on the base of glassy carbon electrode modified with ds-DNA and horseradishperoxidase (HRP). Phenothiazine dyes Methylene Blue and Methylene Green were used aselectrochemical markers for the detection of sulfonamide and anthracycline preparationsable to interact with DNA. The biosensor signal related to HRP oxidation of the markersdepends on the relation between their bonded and readily oxidized forms which depends onthe nature and concentration of pharmaceuticals. Sulfonamides diminish surfaceconcentration of MB accessible for HRP reaction whereas anthracyclines releaseintercalated marker and increase the signal. The DNA-HRP sensor developed makes itpossible to detect down to 0.002 nmol L-1 of sulfamethoxazole, 0.1 nmol L-1 of sulfadiazine,0.01 nmol L-1 of sulfamethazine, 0.1 nmol L-1 of sulfaguanine, 0.05 μmol L-1 of rubomycinand 0.08 μmol L-1 of doxorubicin.
Keywords: DNA sensor; affinity biosensor; horseradish peroxidase; sulfonamide determination; anthracycline determination DNA sensor; affinity biosensor; horseradish peroxidase; sulfonamide determination; anthracycline determination
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Evtugyn, G.A.; Goldfarb, O.E.; Budnikov, H.C.; Ivanov, A.N.; Vinter, V.G. Amperometric DNA-Peroxidase Sensor for the Detection of Pharmaceutical Preparations. Sensors 2005, 5, 364-376.

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