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Development of a DNA Sensor Based on Alkanethiol Self- Assembled Monolayer-Modified Electrodes
Departamento de Química Analítica, Facultad de Ciencias Químicas, Universidad Complutense de Madrid, E-28040 - Madrid, Spain
* Author to whom correspondence should be addressed.
Received: 26 January 2005; Accepted: 3 July 2005 / Published: 14 November 2005
Abstract: An electrochemical DNA biosensor based on recognition of double or singlestranded DNA (ds-DNA/ss-DNA) immobilised on a self-assembled modified gold electrodeis presented for denaturalisation and hybridisation detection. DNA is covalently bond on aself assembled 3-mercaptopropionic acid monolayer by using water soluble N-3-(dimethylaminopropyl)-N´ethylcarbodiimide hydrochloride (EDC) and Nhydroxisulfosuccinimide(NHSS) as linkers. The interaction between the immobilised DNAand methylene blue (MB) is investigated using square wave voltammetry (SWV). Theincrease or diminution of peak currents of the MB upon the hybridisation or denaturalisationevent at the modified electrode surface is studied.
Keywords: Calf thymus DNA; self-assembled monolayers; hybridization; denaturation; methylene blue
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Loaiza, Ó.A.; Campuzano, S.; López-Berlanga, M.; Pedrero, M.; Pingarrón, J.M. Development of a DNA Sensor Based on Alkanethiol Self- Assembled Monolayer-Modified Electrodes. Sensors 2005, 5, 344-363.
Loaiza ÓA, Campuzano S, López-Berlanga M, Pedrero M, Pingarrón JM. Development of a DNA Sensor Based on Alkanethiol Self- Assembled Monolayer-Modified Electrodes. Sensors. 2005; 5(6):344-363.
Loaiza, Óscar A.; Campuzano, Susana; López-Berlanga, María; Pedrero, María; Pingarrón, José M. 2005. "Development of a DNA Sensor Based on Alkanethiol Self- Assembled Monolayer-Modified Electrodes." Sensors 5, no. 6: 344-363.