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Sensors 2005, 5(6), 344-363; doi:10.3390/s5060344

Development of a DNA Sensor Based on Alkanethiol Self- Assembled Monolayer-Modified Electrodes

Departamento de Química Analítica, Facultad de Ciencias Químicas, Universidad Complutense de Madrid, E-28040 - Madrid, Spain
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Received: 26 January 2005 / Accepted: 3 July 2005 / Published: 14 November 2005
(This article belongs to the Special Issue DNA Based Sensors)
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Abstract

An electrochemical DNA biosensor based on recognition of double or singlestranded DNA (ds-DNA/ss-DNA) immobilised on a self-assembled modified gold electrodeis presented for denaturalisation and hybridisation detection. DNA is covalently bond on aself assembled 3-mercaptopropionic acid monolayer by using water soluble N-3-(dimethylaminopropyl)-N´ethylcarbodiimide hydrochloride (EDC) and Nhydroxisulfosuccinimide(NHSS) as linkers. The interaction between the immobilised DNAand methylene blue (MB) is investigated using square wave voltammetry (SWV). Theincrease or diminution of peak currents of the MB upon the hybridisation or denaturalisationevent at the modified electrode surface is studied. View Full-Text
Keywords: Calf thymus DNA; self-assembled monolayers; hybridization; denaturation; methylene blue Calf thymus DNA; self-assembled monolayers; hybridization; denaturation; methylene blue
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Loaiza, Ó.A.; Campuzano, S.; López-Berlanga, M.; Pedrero, M.; Pingarrón, J.M. Development of a DNA Sensor Based on Alkanethiol Self- Assembled Monolayer-Modified Electrodes. Sensors 2005, 5, 344-363.

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