Sensors 2005, 5(11), 473-487; doi:10.3390/s5110473

Brain Tissue Oxygen: In Vivo Monitoring with Carbon Paste Electrodes

Department of Pharmacology, Conway Institute of Biomolecular & Biomedical Research, University College Dublin, Belfield, Dublin 4, Ireland
* Author to whom correspondence should be addressed.
Received: 28 April 2005; Accepted: 1 July 2005 / Published: 16 November 2005
(This article belongs to the Special Issue Carbon Paste Electrodes)
PDF Full-text Download PDF Full-Text [242 KB, uploaded 20 June 2008 16:51 CEST]
Abstract: In this communication we review selected experiments involving the use ofcarbon paste electrodes (CPEs) to monitor and measure brain tissue O2 levels in awakefreely-moving animals. Simultaneous measurements of rCBF were performed using the H2clearance technique. Voltammetric techniques used include both differential pulse (O2) andconstant potential amperometry (rCBF). Mild hypoxia and hyperoxia produced rapidchanges (decrease and increase respectively) in the in vivo O2 signal. Neuronal activation(tail pinch and stimulated grooming) produced similar increases in both O2 and rCBFindicating that CPE O2 currents provide an index of increases in rCBF when such increasesexceed O2 utilization. Saline injection produced a transient increase in the O2 signal whilechloral hydrate produced slower more long-lasting changes that accompanied the behavioralchanges associated with anaesthesia. Acetazolamide increased O2 levels through an increasein rCBF.
Keywords: In-vivo voltammetry; cerebral blood flow; hypoxia and hyperoxia; neuronal activation; chloral hydrate anaesthesia; acetazolamide

Article Statistics

Load and display the download statistics.

Citations to this Article

Cite This Article

MDPI and ACS Style

Bolger, F.B.; Lowry, J.P. Brain Tissue Oxygen: In Vivo Monitoring with Carbon Paste Electrodes. Sensors 2005, 5, 473-487.

AMA Style

Bolger FB, Lowry JP. Brain Tissue Oxygen: In Vivo Monitoring with Carbon Paste Electrodes. Sensors. 2005; 5(11):473-487.

Chicago/Turabian Style

Bolger, Fiachra B.; Lowry, John P. 2005. "Brain Tissue Oxygen: In Vivo Monitoring with Carbon Paste Electrodes." Sensors 5, no. 11: 473-487.

Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert