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Sensors 2017, 17(7), 1506; doi:10.3390/s17071506

Tuning the Sensitivity of the PDR5 Promoter-Based Detection of Diclofenac in Yeast Biosensors

Institute of Genetics, Technische Universität Dresden, 01062 Dresden, Germany
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Received: 23 May 2017 / Revised: 20 June 2017 / Accepted: 21 June 2017 / Published: 26 June 2017
(This article belongs to the Special Issue Whole Cell-Based Biosensors and Application)
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Abstract

The commonly used drug diclofenac is an important environmental anthropogenic pollutant. Currently, detection of diclofenac is mainly based on chemical and physical methods. Here we describe a yeast biosensor that drives the diclofenac-dependent expression of a recombinant fluorescent protein from the authentic promoter of the PDR5 gene. This key component of the pleiotropic drug response encodes a multidrug transporter that is involved in cellular detoxification. We analyse the effects on diclofenac sensitivity of artificial PDR5 promoter derivatives in wild-type and various yeast mutant strains. This approach enabled us to generate sensor strains with elevated drug sensitivity. View Full-Text
Keywords: diclofenac; PDR5; yeast; biosensor diclofenac; PDR5; yeast; biosensor
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Schuller, A.; Rödel, G.; Ostermann, K. Tuning the Sensitivity of the PDR5 Promoter-Based Detection of Diclofenac in Yeast Biosensors. Sensors 2017, 17, 1506.

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