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Sensors 2017, 17(7), 1603; doi:10.3390/s17071603

Microfluidic Platform for the Long-Term On-Chip Cultivation of Mammalian Cells for Lab-On-A-Chip Applications

1
Institute for Microsensors, -actuators and -systems (IMSAS), University of Bremen, 28359 Bremen, Germany
2
Microsystems Center Bremen (MCB), University of Bremen, 28359 Bremen, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Yong Liu
Received: 30 May 2017 / Revised: 4 July 2017 / Accepted: 4 July 2017 / Published: 10 July 2017
(This article belongs to the Special Issue Whole Cell-Based Biosensors and Application)
View Full-Text   |   Download PDF [11113 KB, uploaded 13 July 2017]   |  

Abstract

Lab-on-a-Chip (LoC) applications for the long-term analysis of mammalian cells are still very rare due to the lack of convenient cell cultivation devices. The difficulties are the integration of suitable supply structures, the need of expensive equipment like an incubator and sophisticated pumps as well as the choice of material. The presented device is made out of hard, but non-cytotoxic materials (silicon and glass) and contains two vertical arranged membranes out of hydrogel. The porous membranes are used to separate the culture chamber from two supply channels for gases and nutrients. The cells are fed continuously by diffusion through the membranes without the need of an incubator and low requirements on the supply of medium to the assembly. The diffusion of oxygen is modelled in order to find the optimal dimensions of the chamber. The chip is connected via 3D-printed holders to the macroscopic world. The holders are coated with Parlyene C to ensure that only biocompatible materials are in contact with the culture medium. The experiments with MDCK-cells show the successful seeding inside the chip, culturing and passaging. Consequently, the presented platform is a step towards Lab-on-a-Chip applications that require long-term cultivation of mammalian cells. View Full-Text
Keywords: Lab-on-a-Chip; hydrogel; cell cultivation; MDCK; diffusion model; parylene Lab-on-a-Chip; hydrogel; cell cultivation; MDCK; diffusion model; parylene
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Bunge, F.; Driesche, S.; Vellekoop, M.J. Microfluidic Platform for the Long-Term On-Chip Cultivation of Mammalian Cells for Lab-On-A-Chip Applications. Sensors 2017, 17, 1603.

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