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Sensors 2017, 17(4), 856; doi:10.3390/s17040856

Easy-to-Fabricate and High-Sensitivity LSPR Type Specific Protein Detection Sensor Using AAO Nano-Pore Size Control

1
School of Electronics Engineering, College of IT Engineering, Kyungpook National University, 1370 Sankyuk-dong, Bukgu, 702-701 Daegu, Korea
2
Division of Advanced Research and Development, SINOKOR, 12 Seongseogongdanbuk-ro 43-gil, Dalseo-gu, Daegu 704-920, Korea
3
Division of Computer and Electronic Engineering, Pusan University of Foreign studies, 65 Namsan-dong, Geumjeong-gu, 608-738 Busan, Korea
4
Department of Electronics Engineering, Kyungil University, Hayang-up, Gyeongsang buk-do 712-702, Korea
*
Author to whom correspondence should be addressed.
Received: 28 February 2017 / Revised: 11 April 2017 / Accepted: 12 April 2017 / Published: 13 April 2017
(This article belongs to the Section Biosensors)
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Abstract

In this study, we developed a pore size/pore area-controlled optical biosensor-based anodic aluminum oxide (AAO) nanostructure. As the pore size of AAO increases, the unit cell of AAO increases, which also increases the non-pore area to which the antibody binds. The increase in the number of antibodies immobilized on the surface of the AAO enables effective detection of trace amounts of antigen, because increased antigen-antibody bonding results in a larger surface refractive index change. High sensitivity was thus achieved through amplification of the interference wave of two vertically-incident reflected waves through the localized surface plasmon resonance phenomenon. The sensitivity of the fabricated sensor was evaluated by measuring the change in wavelength with the change in the refractive index of the device surface, and sensitivity was increased with increasing pore-size and non-pore area. The sensitivity of the fabricated sensor was improved and up to 11.8 ag/mL serum amyloid A1 antigen was detected. In addition, the selectivity of the fabricated sensor was confirmed through a reaction with a heterogeneous substance, C-reactive protein antigen. By using hard anodization during fabrication of the AAO, the fabrication time of the device was reduced and the AAO chip was fabricated quickly and easily. View Full-Text
Keywords: localized surface plasmon resonance (LSPR); optical interferometry; anodic aluminum oxide (AAO); pore-size; pore-area; serum amyloid A1 (SAA1); optical-biosensor; C-reactive protein (CRP) localized surface plasmon resonance (LSPR); optical interferometry; anodic aluminum oxide (AAO); pore-size; pore-area; serum amyloid A1 (SAA1); optical-biosensor; C-reactive protein (CRP)
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Kim, S.-W.; Lee, J.-S.; Lee, S.-W.; Kang, B.-H.; Kwon, J.-B.; Kim, O.-S.; Kim, J.-S.; Kim, E.-S.; Kwon, D.-H.; Kang, S.-W. Easy-to-Fabricate and High-Sensitivity LSPR Type Specific Protein Detection Sensor Using AAO Nano-Pore Size Control. Sensors 2017, 17, 856.

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