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Sensors 2017, 17(4), 831; doi:10.3390/s17040831

Miniaturized Real-Time PCR on a Q3 System for Rapid KRAS Genotyping

1
Institute of Neurological Sciences, Italian National Research Council, Via Paolo Gaifami, 18-95125 Catania, Italy
2
STMicroelectronics, Stradale Primosole, 50-95121 Catania, Italy
*
Authors to whom correspondence should be addressed.
Academic Editor: Alexander Star
Received: 13 March 2017 / Revised: 29 March 2017 / Accepted: 5 April 2017 / Published: 11 April 2017
(This article belongs to the Section Biosensors)
View Full-Text   |   Download PDF [2097 KB, uploaded 11 April 2017]   |  

Abstract

Colorectal cancer (CRC) is an aggressive human malignancy with a complex genomic landscape harboring KRAS mutations. In 40%–60% of patients with CRC, constantly active KRAS proteins affect the prognosis, surgical strategy, and clinical benefit from therapy with anti-epidermal growth factor receptor (EGFR) agents. For this reason, there is a greater demand for minimally-invasive diagnostic devices to characterize the genetic pattern and prevent the acquired mechanism to drug resistance. The rapid developments in cutting-edge diagnostic techniques are expected to play a growing role in medicine and represent an attractive promise to identify potential responders to personalized medicine. Here we propose a new method to simultaneously detect the main KRAS mutations on the portable real-time PCR Q3 platform. This platform is based on hybrid silicon-plastic technology implemented in a miniaturized chip able to achieve a sample-in answer-out rapid analysis, allowing a new approach to genetic counseling and testing. View Full-Text
Keywords: qPCR; Genome-based test; diagnostics; colorectal cancer qPCR; Genome-based test; diagnostics; colorectal cancer
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Guarnaccia, M.; Iemmolo, R.; Petralia, S.; Conoci, S.; Cavallaro, S. Miniaturized Real-Time PCR on a Q3 System for Rapid KRAS Genotyping. Sensors 2017, 17, 831.

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