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Sensors 2017, 17(3), 520; doi:10.3390/s17030520

Development of Fluorescent FRET Probes for “Off-On” Detection of L-Cysteine Based on Gold Nanoparticles and Porous Silicon Nanoparticles in Ethanol Solution

1
School of Physical Science and Technology, Xinjiang University, Urumqi 830046, China
2
College of Information Science and Engineering, Xinjiang University, Urumqi 830046, China
*
Author to whom correspondence should be addressed.
Academic Editors: Nathan Lindquist, Nathan Wittenberg and Sang-Hyun Oh
Received: 10 January 2017 / Revised: 20 February 2017 / Accepted: 28 February 2017 / Published: 5 March 2017
(This article belongs to the Special Issue Micro and Nanofabrication Technologies for Biosensors)
View Full-Text   |   Download PDF [2470 KB, uploaded 5 March 2017]   |  

Abstract

A new type of fluorescence “off-on” probe was designed for L-Cysteine (L-Cys) based on the fluorescence resonance energy transfer (FRET) between negatively charged amino-capped porous silicon nanoparticles (SiNPs) and positively charged citrate-stabilized Au nanoparticles (AuNPs). In this proposed FRET immunosensor, novel water-soluble amino-conjugated porous SiNPs in ethanol with excellent photoluminescence properties act as the energy donor. Excellent quenching efficiency between SiNPs-ethanol and citrate-stabilized AuNPs by electrostatic interaction via FRET provides an ideal “off-state” (turn-off). The addition of L-Cys leads to releasing the adsorbed AuNPs from the surface of SiNPs and hence the fluorescence emission of SiNPs-ethanol is restored (turn-on), which means the coordination ability of the thiols with AuNPs is stronger than that of the electrostatic interaction. The fluorescence intensity of SiNPs-AuNPs in ethanol is sensitive to L-Cys, and such a restored fluorescence is proportional to the concentration of L-Cys. The method will broadly benefit the development of a new thiol biosensor based on nanostructured porous materials, and the proposed procedure is also expected to develop a variety of functional nanoparticles to form other novel kinds of nanosensors. View Full-Text
Keywords: “off-on” fluorescence biosensor; porous SiNPs; ethanol solution; AuNPs; L-Cysteine “off-on” fluorescence biosensor; porous SiNPs; ethanol solution; AuNPs; L-Cysteine
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Zhang, H.; Jia, Z. Development of Fluorescent FRET Probes for “Off-On” Detection of L-Cysteine Based on Gold Nanoparticles and Porous Silicon Nanoparticles in Ethanol Solution. Sensors 2017, 17, 520.

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