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Sensors 2017, 17(3), 520; doi:10.3390/s17030520

Development of Fluorescent FRET Probes for “Off-On” Detection of L-Cysteine Based on Gold Nanoparticles and Porous Silicon Nanoparticles in Ethanol Solution

School of Physical Science and Technology, Xinjiang University, Urumqi 830046, China
College of Information Science and Engineering, Xinjiang University, Urumqi 830046, China
Author to whom correspondence should be addressed.
Academic Editors: Nathan Lindquist, Nathan Wittenberg and Sang-Hyun Oh
Received: 10 January 2017 / Revised: 20 February 2017 / Accepted: 28 February 2017 / Published: 5 March 2017
(This article belongs to the Special Issue Micro and Nanofabrication Technologies for Biosensors)
View Full-Text   |   Download PDF [2470 KB, uploaded 5 March 2017]   |  


A new type of fluorescence “off-on” probe was designed for L-Cysteine (L-Cys) based on the fluorescence resonance energy transfer (FRET) between negatively charged amino-capped porous silicon nanoparticles (SiNPs) and positively charged citrate-stabilized Au nanoparticles (AuNPs). In this proposed FRET immunosensor, novel water-soluble amino-conjugated porous SiNPs in ethanol with excellent photoluminescence properties act as the energy donor. Excellent quenching efficiency between SiNPs-ethanol and citrate-stabilized AuNPs by electrostatic interaction via FRET provides an ideal “off-state” (turn-off). The addition of L-Cys leads to releasing the adsorbed AuNPs from the surface of SiNPs and hence the fluorescence emission of SiNPs-ethanol is restored (turn-on), which means the coordination ability of the thiols with AuNPs is stronger than that of the electrostatic interaction. The fluorescence intensity of SiNPs-AuNPs in ethanol is sensitive to L-Cys, and such a restored fluorescence is proportional to the concentration of L-Cys. The method will broadly benefit the development of a new thiol biosensor based on nanostructured porous materials, and the proposed procedure is also expected to develop a variety of functional nanoparticles to form other novel kinds of nanosensors. View Full-Text
Keywords: “off-on” fluorescence biosensor; porous SiNPs; ethanol solution; AuNPs; L-Cysteine “off-on” fluorescence biosensor; porous SiNPs; ethanol solution; AuNPs; L-Cysteine

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Zhang, H.; Jia, Z. Development of Fluorescent FRET Probes for “Off-On” Detection of L-Cysteine Based on Gold Nanoparticles and Porous Silicon Nanoparticles in Ethanol Solution. Sensors 2017, 17, 520.

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