Microshell Arrays Enhanced Sensitivity in Detection of Specific Antibody for Reduced Graphene Oxide Optical Sensor
AbstractProtein-protein interactions play an important role in the investigation of biomolecules. In this paper, we reported on the use of a reduced graphene oxide microshell (RGOM)-based optical biosensor for the determination of goat anti-rabbit IgG. The biosensor was prepared through a self-assembly of monolayers of monodisperse polystyrene microspheres, combined with a high-temperature reduction, in order to decorate the RGOM with rabbit IgG. The periodic microshells allowed a simpler functionalization and modification of RGOM with bioreceptor units, than reduced graphene oxide (RGO). With additional antibody-antigen binding, the RGOM-based biosensor achieved better real-time and label-free detection. The RGOM-based biosensor presented a more satisfactory response to goat anti-rabbit IgG than the RGO-based biosensor. This method is promising for immobilizing biomolecules on graphene surfaces and for the fabrication of biosensors with enhanced sensitivity. View Full-Text
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Jiang, W.-S.; Xin, W.; Chen, S.-N.; Li, C.-B.; Gao, X.-G.; Pan, L.-T.; Liu, Z.-B.; Tian, J.-G. Microshell Arrays Enhanced Sensitivity in Detection of Specific Antibody for Reduced Graphene Oxide Optical Sensor. Sensors 2017, 17, 221.
Jiang W-S, Xin W, Chen S-N, Li C-B, Gao X-G, Pan L-T, Liu Z-B, Tian J-G. Microshell Arrays Enhanced Sensitivity in Detection of Specific Antibody for Reduced Graphene Oxide Optical Sensor. Sensors. 2017; 17(2):221.Chicago/Turabian Style
Jiang, Wen-Shuai; Xin, Wei; Chen, Shao-Nan; Li, Cun-Bo; Gao, Xiao-Guang; Pan, Lei-Ting; Liu, Zhi-Bo; Tian, Jian-Guo. 2017. "Microshell Arrays Enhanced Sensitivity in Detection of Specific Antibody for Reduced Graphene Oxide Optical Sensor." Sensors 17, no. 2: 221.