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Sensors 2018, 18(1), 36; https://doi.org/10.3390/s18010036

Theoretical and Experimental Comparison of Different Formats of Immunochromatographic Serodiagnostics

A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, Moscow 119071, Russia
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Received: 10 November 2017 / Revised: 20 December 2017 / Accepted: 22 December 2017 / Published: 25 December 2017
(This article belongs to the Special Issue Biosensors for Antibody Detection)
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Abstract

In this study, a comparative theoretical and experimental analysis of two immuno-chromatographic serodiagnostics schemes, which differ in the immobilization of immunoreagents and the order of the formation of immune complexes, is performed. Based on the theoretical models, the assays are characterized to determine which scheme has a higher quantity of the detected complex and thus ensures the sensitivity of the analysis. The results show that for the effective detection of low-affinity antibodies, the scheme involving the immobilization of the antigen on gold nanoparticles and the antibody-binding protein on the test strip was more sensitive than the predominantly used scheme, which inverts the immunoreagents’ locations. The theoretical predictions were confirmed by the experimental testing of sera collected from tuberculosis patients. View Full-Text
Keywords: lateral flow immunoassay; detection of antibodies; mathematic simulation; tuberculosis lateral flow immunoassay; detection of antibodies; mathematic simulation; tuberculosis
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Sotnikov, D.V.; Zherdev, A.V.; Dzantiev, B.B. Theoretical and Experimental Comparison of Different Formats of Immunochromatographic Serodiagnostics. Sensors 2018, 18, 36.

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