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Sensors 2016, 16(4), 574; doi:10.3390/s16040574

Protein Chips for Detection of Salmonella spp. from Enrichment Culture

1
CNR-ISPA, Institute of Sciences of Food Productions, via Monteroni km 7, 73100 Lecce, Italy
2
Biotecgen, c/o Department of Biological and Environmental Sciences and Technologies, University of Salento, 73100 Lecce, Italy
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Benoit Piro
Received: 18 January 2016 / Revised: 18 April 2016 / Accepted: 19 April 2016 / Published: 22 April 2016
(This article belongs to the Section Biosensors)
View Full-Text   |   Download PDF [2381 KB, uploaded 22 April 2016]   |  

Abstract

Food pathogens are the cause of foodborne epidemics, therefore there is a need to detect the pathogens in food productions rapidly. A pre-enrichment culture followed by selective agar plating are standard detection methods. Molecular methods such as qPCR have provided a first rapid protocol for detection of pathogens within 24 h of enrichment culture. Biosensors also may provide a rapid tool to individuate a source of Salmonella contamination at early times of pre-enrichment culture. Forty mL of Salmonella spp. enrichment culture were processed by immunoseparation using the Pathatrix, as in AFNOR validated qPCR protocols. The Salmonella biosensor combined with immunoseparation showed a limit of detection of 100 bacteria/40 mL, with a 400 fold increase to previous results. qPCR analysis requires processing of bead-bound bacteria with lysis buffer and DNA clean up, with a limit of detection of 2 cfu/50 μL. Finally, a protein chip was developed and tested in screening and identification of 5 common pathogen species, Salmonella spp., E. coli, S. aureus, Campylobacter spp. and Listeria spp. The protein chip, with high specificity in species identification, is proposed to be integrated into a Lab-on-Chip system, for rapid and reproducible screening of Salmonella spp. and other pathogen species contaminating food productions. View Full-Text
Keywords: antibody; biosensors; detection; food pathogens; identification; labelling; Salmonella spp. antibody; biosensors; detection; food pathogens; identification; labelling; Salmonella spp.
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Poltronieri, P.; Cimaglia, F.; De Lorenzis, E.; Chiesa, M.; Mezzolla, V.; Reca, I.B. Protein Chips for Detection of Salmonella spp. from Enrichment Culture. Sensors 2016, 16, 574.

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