Protein Chips for Detection of Salmonella spp. from Enrichment Culture
AbstractFood pathogens are the cause of foodborne epidemics, therefore there is a need to detect the pathogens in food productions rapidly. A pre-enrichment culture followed by selective agar plating are standard detection methods. Molecular methods such as qPCR have provided a first rapid protocol for detection of pathogens within 24 h of enrichment culture. Biosensors also may provide a rapid tool to individuate a source of Salmonella contamination at early times of pre-enrichment culture. Forty mL of Salmonella spp. enrichment culture were processed by immunoseparation using the Pathatrix, as in AFNOR validated qPCR protocols. The Salmonella biosensor combined with immunoseparation showed a limit of detection of 100 bacteria/40 mL, with a 400 fold increase to previous results. qPCR analysis requires processing of bead-bound bacteria with lysis buffer and DNA clean up, with a limit of detection of 2 cfu/50 μL. Finally, a protein chip was developed and tested in screening and identification of 5 common pathogen species, Salmonella spp., E. coli, S. aureus, Campylobacter spp. and Listeria spp. The protein chip, with high specificity in species identification, is proposed to be integrated into a Lab-on-Chip system, for rapid and reproducible screening of Salmonella spp. and other pathogen species contaminating food productions. View Full-Text
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Poltronieri, P.; Cimaglia, F.; De Lorenzis, E.; Chiesa, M.; Mezzolla, V.; Reca, I.B. Protein Chips for Detection of Salmonella spp. from Enrichment Culture. Sensors 2016, 16, 574.
Poltronieri P, Cimaglia F, De Lorenzis E, Chiesa M, Mezzolla V, Reca IB. Protein Chips for Detection of Salmonella spp. from Enrichment Culture. Sensors. 2016; 16(4):574.Chicago/Turabian Style
Poltronieri, Palmiro; Cimaglia, Fabio; De Lorenzis, Enrico; Chiesa, Maurizio; Mezzolla, Valeria; Reca, Ida B. 2016. "Protein Chips for Detection of Salmonella spp. from Enrichment Culture." Sensors 16, no. 4: 574.
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