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Sensors 2016, 16(11), 1909; doi:10.3390/s16111909

Optical Aptamer Probes of Fluorescent Imaging to Rapid Monitoring of Circulating Tumor Cell

1
Preclinical Research Center, Biomedical Research Institute, Seoul National University Bundang Hospital, 82, Gumi-ro 173beon-gil, Bundang-gu, Seongnam-si, Gyeonggi-do 13620, Korea
2
The Institute for the 3Rs, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Korea
3
Department of Surgery, Seoul National University Bundang Hospital, 82, Gumi-ro 173beon-gil, Bundang-gu, Seongnam-si, Gyeonggi-do 13620, Korea
4
Asan Institute for Life Sciences, Asan Medical Center, Seoul, Department of Medicine, University of Ulsan College of Medicine, 43 gil Olympic-ro, Pungnap dong, Songpa gu, Seoul 138-736, Korea
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Huangxian Ju
Received: 5 August 2016 / Revised: 2 November 2016 / Accepted: 2 November 2016 / Published: 23 November 2016
(This article belongs to the Special Issue Nanobiosensing for Sensors)
View Full-Text   |   Download PDF [2729 KB, uploaded 23 November 2016]   |  

Abstract

Fluorescence detecting of exogenous EpCAM (epithelial cell adhesion molecule) or muc1 (mucin1) expression correlated to cancer metastasis using nanoparticles provides pivotal information on CTC (circulating tumor cell) occurrence in a noninvasive tool. In this study, we study a new skill to detect extracellular EpCAM/muc1 using quantum dot-based aptamer beacon (QD-EpCAM/muc1 ALB (aptamer linker beacon). The QD-EpCAM/muc1 ALB was designed using QDs (quantum dots) and probe. The EpCAM/muc1-targeting aptamer contains a Ep-CAM/muc1 binding sequence and BHQ1 (black hole quencher 1) or BHQ2 (black hole quencher2). In the absence of target EpCAM/muc1, the QD-EpCAM/muc1 ALB forms a partial duplex loop-like aptamer beacon and remained in quenched state because the BHQ1/2 quenches the fluorescence signal-on of the QD-EpCAM/muc1 ALB. The binding of EpCAM/muc1 of CTC to the EpCAM/muc1 binding aptamer sequence of the EpCAM/muc1-targeting oligonucleotide triggered the dissociation of the BHQ1/2 quencher and subsequent signal-on of a green/red fluorescence signal. Furthermore, acute inflammation was stimulated by trigger such as caerulein in vivo, which resulted in increased fluorescent signal of the cy5.5-EpCAM/muc1 ALB during cancer metastasis due to exogenous expression of EpCAM/muc1 in Panc02-implanted mouse model. View Full-Text
Keywords: EpCAM; aptamer; ALB; metastasis; molecular beacon EpCAM; aptamer; ALB; metastasis; molecular beacon
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MDPI and ACS Style

Hwang, J.Y.; Kim, S.T.; Han, H.-S.; Kim, K.; Han, J.S. Optical Aptamer Probes of Fluorescent Imaging to Rapid Monitoring of Circulating Tumor Cell. Sensors 2016, 16, 1909.

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