Next Article in Journal
Accelerating Families of Fuzzy K-Means Algorithms for Vector Quantization Codebook Design
Next Article in Special Issue
Potentiometric Aptasensing of Vibrio alginolyticus Based on DNA Nanostructure-Modified Magnetic Beads
Previous Article in Journal
Identification-While-Scanning of a Multi-Aircraft Formation Based on Sparse Recovery for Narrowband Radar
Previous Article in Special Issue
Highly Sensitive and Practical Detection of Plant Viruses via Electrical Impedance of Droplets on Textured Silicon-Based Devices
Article Menu

Export Article

Open AccessArticle
Sensors 2016, 16(11), 1909; doi:10.3390/s16111909

Optical Aptamer Probes of Fluorescent Imaging to Rapid Monitoring of Circulating Tumor Cell

Preclinical Research Center, Biomedical Research Institute, Seoul National University Bundang Hospital, 82, Gumi-ro 173beon-gil, Bundang-gu, Seongnam-si, Gyeonggi-do 13620, Korea
The Institute for the 3Rs, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Korea
Department of Surgery, Seoul National University Bundang Hospital, 82, Gumi-ro 173beon-gil, Bundang-gu, Seongnam-si, Gyeonggi-do 13620, Korea
Asan Institute for Life Sciences, Asan Medical Center, Seoul, Department of Medicine, University of Ulsan College of Medicine, 43 gil Olympic-ro, Pungnap dong, Songpa gu, Seoul 138-736, Korea
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Academic Editor: Huangxian Ju
Received: 5 August 2016 / Revised: 2 November 2016 / Accepted: 2 November 2016 / Published: 23 November 2016
(This article belongs to the Special Issue Nanobiosensing for Sensors)
View Full-Text   |   Download PDF [2729 KB, uploaded 23 November 2016]   |  


Fluorescence detecting of exogenous EpCAM (epithelial cell adhesion molecule) or muc1 (mucin1) expression correlated to cancer metastasis using nanoparticles provides pivotal information on CTC (circulating tumor cell) occurrence in a noninvasive tool. In this study, we study a new skill to detect extracellular EpCAM/muc1 using quantum dot-based aptamer beacon (QD-EpCAM/muc1 ALB (aptamer linker beacon). The QD-EpCAM/muc1 ALB was designed using QDs (quantum dots) and probe. The EpCAM/muc1-targeting aptamer contains a Ep-CAM/muc1 binding sequence and BHQ1 (black hole quencher 1) or BHQ2 (black hole quencher2). In the absence of target EpCAM/muc1, the QD-EpCAM/muc1 ALB forms a partial duplex loop-like aptamer beacon and remained in quenched state because the BHQ1/2 quenches the fluorescence signal-on of the QD-EpCAM/muc1 ALB. The binding of EpCAM/muc1 of CTC to the EpCAM/muc1 binding aptamer sequence of the EpCAM/muc1-targeting oligonucleotide triggered the dissociation of the BHQ1/2 quencher and subsequent signal-on of a green/red fluorescence signal. Furthermore, acute inflammation was stimulated by trigger such as caerulein in vivo, which resulted in increased fluorescent signal of the cy5.5-EpCAM/muc1 ALB during cancer metastasis due to exogenous expression of EpCAM/muc1 in Panc02-implanted mouse model. View Full-Text
Keywords: EpCAM; aptamer; ALB; metastasis; molecular beacon EpCAM; aptamer; ALB; metastasis; molecular beacon

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Hwang, J.Y.; Kim, S.T.; Han, H.-S.; Kim, K.; Han, J.S. Optical Aptamer Probes of Fluorescent Imaging to Rapid Monitoring of Circulating Tumor Cell. Sensors 2016, 16, 1909.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top