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Sensors 2016, 16(11), 1946; doi:10.3390/s16111946

Highly Sensitive and Practical Detection of Plant Viruses via Electrical Impedance of Droplets on Textured Silicon-Based Devices

1
CNR NANOTEC—Istituto di Nanotecnologia, Via Amendola 122, Bari 70126, Italy
2
CNR IPSP—Istituto per la Protezione Sostenibile delle Piante, UoS Bari, Via Amendola 165, Bari 70126, Italy
3
Dipartimento di Chimica, Università degli Studi di Bari, Via Orabona 4, Bari 70125, Italy
4
CNR ICCOM—Istituto di Chimica dei Composti OrganoMetallici, Via Orabona 4, Bari 70125, Italy
5
Dipartimento Interateneo di Fisica, Università degli Studi di Bari, Via Orabona 4, Bari 70125, Italy
*
Author to whom correspondence should be addressed.
Academic Editor: Huangxian Ju
Received: 16 September 2016 / Revised: 8 November 2016 / Accepted: 11 November 2016 / Published: 18 November 2016
(This article belongs to the Special Issue Nanobiosensing for Sensors)
View Full-Text   |   Download PDF [3169 KB, uploaded 18 November 2016]   |  

Abstract

Early diagnosis of plant virus infections before the disease symptoms appearance may represent a significant benefit in limiting disease spread by a prompt application of appropriate containment steps. We propose a label-free procedure applied on a device structure where the electrical signal transduction is evaluated via impedance spectroscopy techniques. The device consists of a droplet suspension embedding two representative purified plant viruses i.e., Tomato mosaic virus and Turnip yellow mosaic virus, put in contact with a highly hydrophobic plasma textured silicon surface. Results show a high sensitivity of the system towards the virus particles with an interestingly low detection limit, from tens to hundreds of attomolar corresponding to pg/mL of sap, which refers, in the infection time-scale, to a concentration of virus particles in still-symptomless plants. Such a threshold limit, together with an envisaged engineering of an easily manageable device, compared to more sophisticated apparatuses, may contribute in simplifying the in-field plant virus diagnostics. View Full-Text
Keywords: plant viruses; EIS; label-free detection; ToMV; TYMV; droplet-based device; surface texturing plant viruses; EIS; label-free detection; ToMV; TYMV; droplet-based device; surface texturing
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Ambrico, M.; Ambrico, P.F.; Minafra, A.; De Stradis, A.; Vona, D.; Cicco, S.R.; Palumbo, F.; Favia, P.; Ligonzo, T. Highly Sensitive and Practical Detection of Plant Viruses via Electrical Impedance of Droplets on Textured Silicon-Based Devices. Sensors 2016, 16, 1946.

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