Highly Sensitive and Practical Detection of Plant Viruses via Electrical Impedance of Droplets on Textured Silicon-Based Devices
AbstractEarly diagnosis of plant virus infections before the disease symptoms appearance may represent a significant benefit in limiting disease spread by a prompt application of appropriate containment steps. We propose a label-free procedure applied on a device structure where the electrical signal transduction is evaluated via impedance spectroscopy techniques. The device consists of a droplet suspension embedding two representative purified plant viruses i.e., Tomato mosaic virus and Turnip yellow mosaic virus, put in contact with a highly hydrophobic plasma textured silicon surface. Results show a high sensitivity of the system towards the virus particles with an interestingly low detection limit, from tens to hundreds of attomolar corresponding to pg/mL of sap, which refers, in the infection time-scale, to a concentration of virus particles in still-symptomless plants. Such a threshold limit, together with an envisaged engineering of an easily manageable device, compared to more sophisticated apparatuses, may contribute in simplifying the in-field plant virus diagnostics. View Full-Text
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Ambrico, M.; Ambrico, P.F.; Minafra, A.; De Stradis, A.; Vona, D.; Cicco, S.R.; Palumbo, F.; Favia, P.; Ligonzo, T. Highly Sensitive and Practical Detection of Plant Viruses via Electrical Impedance of Droplets on Textured Silicon-Based Devices. Sensors 2016, 16, 1946.
Ambrico M, Ambrico PF, Minafra A, De Stradis A, Vona D, Cicco SR, Palumbo F, Favia P, Ligonzo T. Highly Sensitive and Practical Detection of Plant Viruses via Electrical Impedance of Droplets on Textured Silicon-Based Devices. Sensors. 2016; 16(11):1946.Chicago/Turabian Style
Ambrico, Marianna; Ambrico, Paolo F.; Minafra, Angelantonio; De Stradis, Angelo; Vona, Danilo; Cicco, Stefania R.; Palumbo, Fabio; Favia, Pietro; Ligonzo, Teresa. 2016. "Highly Sensitive and Practical Detection of Plant Viruses via Electrical Impedance of Droplets on Textured Silicon-Based Devices." Sensors 16, no. 11: 1946.
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