Next Article in Journal
Multi-Stage Feature Selection by Using Genetic Algorithms for Fault Diagnosis in Gearboxes Based on Vibration Signal
Next Article in Special Issue
Microfluidic Organ/Body-on-a-Chip Devices at the Convergence of Biology and Microengineering
Previous Article in Journal
“Stable-on-the-Table” Biosensors: Hemoglobin-Poly (Acrylic Acid) Nanogel BioElectrodes with High Thermal Stability and Enhanced Electroactivity
Previous Article in Special Issue
Design and Evaluation of Potentiometric Principles for Bladder Volume Monitoring: A Preliminary Study
Article Menu

Export Article

Open AccessArticle
Sensors 2015, 15(9), 23886-23902; doi:10.3390/s150923886

Enhanced Viability of Endothelial Colony Forming Cells in Fibrin Microbeads for Sensor Vascularization

1
Department of Biomedical Engineering, Wishnick Hall 223, 3255 South Dearborn Street, Chicago, IL 60616, USA
2
Department of Physiology, Faculty of Pharmacy, University of Belgrade, Belgrade 11000, Serbia
3
Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA
4
Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, IN 46201, USA
5
Research Service, Hines Veterans Administration Hospital, Hines, IL 60141, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Jennie B. Leach
Received: 11 August 2015 / Revised: 3 September 2015 / Accepted: 15 September 2015 / Published: 18 September 2015
(This article belongs to the Special Issue Novel Biomaterials and Sensors for Tissue Engineering)
View Full-Text   |   Download PDF [800 KB, uploaded 18 September 2015]   |  

Abstract

Enhanced vascularization at sensor interfaces can improve long-term function. Fibrin, a natural polymer, has shown promise as a biomaterial for sensor coating due to its ability to sustain endothelial cell growth and promote local vascularization. However, the culture of cells, particularly endothelial cells (EC), within 3D scaffolds for more than a few days is challenging due to rapid loss of EC viability. In this manuscript, a robust method for developing fibrin microbead scaffolds for long-term culture of encapsulated ECs is described. Fibrin microbeads are formed using sodium alginate as a structural template. The size, swelling and structural properties of the microbeads were varied with needle gauge and composition and concentration of the pre-gel solution. Endothelial colony-forming cells (ECFCs) were suspended in the fibrin beads and cultured within a perfusion bioreactor system. The perfusion bioreactor enhanced ECFCs viability and genome stability in fibrin beads relative to static culture. Perfusion bioreactors enable 3D culture of ECs within fibrin beads for potential application as a sensor coating. View Full-Text
Keywords: tissue engineering; vascularization; bioreactor; long-term sensors; implantable sensors; fibrin tissue engineering; vascularization; bioreactor; long-term sensors; implantable sensors; fibrin
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Gandhi, J.K.; Zivkovic, L.; Fisher, J.P.; Yoder, M.C.; Brey, E.M. Enhanced Viability of Endothelial Colony Forming Cells in Fibrin Microbeads for Sensor Vascularization. Sensors 2015, 15, 23886-23902.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top