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Open AccessCommunication
Sensors 2015, 15(9), 22547-22560; doi:10.3390/s150922547

Aptameric Recognition-Modulated Electroactivity of Poly(4-Styrenesolfonic Acid)-Doped Polyaniline Films for Single-Shot Detection of Tetrodotoxin

SensorLab, Chemistry Department, University of the Western Cape, P. Bag X17, Bellville, Cape Town 7535, South Africa
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Author to whom correspondence should be addressed.
Academic Editor: Alexander Star
Received: 8 June 2015 / Accepted: 31 August 2015 / Published: 8 September 2015
(This article belongs to the Section Biosensors)
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Abstract

The work being reported is the first electrochemical sensor for tetrodotoxin (TTX). It was developed on a glassy carbon electrodes (C) that was modified with poly(4-styrenesolfonic acid)-doped polyaniline film (PANI/PSSA). An amine-end functionalized TTX-binding aptamer, 5′-NH2-AAAAATTTCACACGGGTGCCTCGGCTGTCC-3′ (NH2-Apt), was grafted via covalent glutaraldehyde (glu) cross-linking. The resulting aptasensor (C//PANI+/PSSA-glu-NH2-Apt) was interrogated by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) in sodium acetate buffer (NaOAc, pH 4.8) before and after 30 min incubation in standard TTX solutions. Both CV and EIS results confirmed that the binding of the analyte to the immobilized aptamer modulated the electrochemical properties of the sensor: particularly the charge transfer resistance (Rct) of the PANI+/PSSA film, which served as a signal reporter. Based on the Rct calibration curve of the TTX aptasensor, the values of the dynamic linear range (DLR), sensitivity and limit of detection (LOD) of the sensor were determined to be 0.23–1.07 ng·mL−1 TTX, 134.88 ± 11.42 Ω·ng·mL−1 and 0.199 ng·mL−1, respectively. Further studies are being planned to improve the DLR as well as to evaluate selectivity and matrix effects in real samples. View Full-Text
Keywords: tetrodotoxin sensor; aptamer; polyaniline; puffer fish tetrodotoxin sensor; aptamer; polyaniline; puffer fish
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Fomo, G.; Waryo, T.T.; Sunday, C.E.; Baleg, A.A.; Baker, P.G.; Iwuoha, E.I. Aptameric Recognition-Modulated Electroactivity of Poly(4-Styrenesolfonic Acid)-Doped Polyaniline Films for Single-Shot Detection of Tetrodotoxin. Sensors 2015, 15, 22547-22560.

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