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Sensors 2015, 15(11), 28244-28256; doi:10.3390/s151128244

A Graphene-Based Biosensing Platform Based on Regulated Release of an Aptameric DNA Biosensor

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1,3
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1
Laboratory of Chemical Genomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, Shenzhen 518055, China
2
The Ministry-Province Jointly Constructed Base for State Key Lab-Shenzhen Key Laboratory of Chemical Biology, the Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China
3
Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Paul E. Laibinis
Received: 12 September 2015 / Revised: 31 October 2015 / Accepted: 4 November 2015 / Published: 9 November 2015
(This article belongs to the Section Biosensors)
View Full-Text   |   Download PDF [406 KB, uploaded 10 November 2015]   |  

Abstract

A novel biosensing platform was developed by integrating an aptamer-based DNA biosensor with graphene oxide (GO) for rapid and facile detection of adenosine triphosphate (ATP, as a model target). The DNA biosensor, which is locked by GO, is designed to contain two sensing modules that include recognition site for ATP and self-replication track that yields the nicking domain for Nt.BbvCI. By taking advantage of the different binding affinity of single-stranded DNA, double-stranded DNA and aptamer-target complex toward GO, the DNA biosensor could be efficiently released from GO in the presence of target with the help of a complementary DNA strand (CPDNA) that partially hybridizes to the DNA biosensor. Then, the polymerization/nicking enzyme synergetic isothermal amplification could be triggered, leading to the synthesis of massive DNA amplicons, thus achieving an enhanced sensitivity with a wide linear dynamic response range of four orders of magnitude and good selectivity. This biosensing strategy expands the applications of GO-DNA nanobiointerfaces in biological sensing, showing great potential in fundamental research and biomedical diagnosis. View Full-Text
Keywords: aptamer; DNA biosensor; graphene oxide; Isothermal amplification aptamer; DNA biosensor; graphene oxide; Isothermal amplification
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Mao, Y.; Chen, Y.; Li, S.; Lin, S.; Jiang, Y. A Graphene-Based Biosensing Platform Based on Regulated Release of an Aptameric DNA Biosensor. Sensors 2015, 15, 28244-28256.

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