A Graphene-Based Biosensing Platform Based on Regulated Release of an Aptameric DNA Biosensor
AbstractA novel biosensing platform was developed by integrating an aptamer-based DNA biosensor with graphene oxide (GO) for rapid and facile detection of adenosine triphosphate (ATP, as a model target). The DNA biosensor, which is locked by GO, is designed to contain two sensing modules that include recognition site for ATP and self-replication track that yields the nicking domain for Nt.BbvCI. By taking advantage of the different binding affinity of single-stranded DNA, double-stranded DNA and aptamer-target complex toward GO, the DNA biosensor could be efficiently released from GO in the presence of target with the help of a complementary DNA strand (CPDNA) that partially hybridizes to the DNA biosensor. Then, the polymerization/nicking enzyme synergetic isothermal amplification could be triggered, leading to the synthesis of massive DNA amplicons, thus achieving an enhanced sensitivity with a wide linear dynamic response range of four orders of magnitude and good selectivity. This biosensing strategy expands the applications of GO-DNA nanobiointerfaces in biological sensing, showing great potential in fundamental research and biomedical diagnosis. View Full-Text
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Mao, Y.; Chen, Y.; Li, S.; Lin, S.; Jiang, Y. A Graphene-Based Biosensing Platform Based on Regulated Release of an Aptameric DNA Biosensor. Sensors 2015, 15, 28244-28256.
Mao Y, Chen Y, Li S, Lin S, Jiang Y. A Graphene-Based Biosensing Platform Based on Regulated Release of an Aptameric DNA Biosensor. Sensors. 2015; 15(11):28244-28256.Chicago/Turabian Style
Mao, Yu; Chen, Yongli; Li, Song; Lin, Shuo; Jiang, Yuyang. 2015. "A Graphene-Based Biosensing Platform Based on Regulated Release of an Aptameric DNA Biosensor." Sensors 15, no. 11: 28244-28256.