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Sensors 2015, 15(1), 331-340; doi:10.3390/s150100331

Surface Plasmon Resonator Using High Sensitive Resonance Telecommunication Wavelengths for DNA Sensors of Mycobacterium Tuberculosis with Thiol-Modified Probes

Department of Electronic Engineering, National Taiwan University of Science and Technology, No. 43, Sec. 4, Keelung Rd., Taipei 10607, Taiwan
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Author to whom correspondence should be addressed.
Received: 22 August 2014 / Accepted: 18 December 2014 / Published: 25 December 2014
(This article belongs to the Special Issue Next-Generation Nucleic Acid Sensors)
View Full-Text   |   Download PDF [440 KB, uploaded 25 December 2014]   |  

Abstract

Various analytes can be verified by surface plasmon resonance, thus continuous improvement of this sensing technology is crucial for better sensing selection and higher sensitivity. The SPR sensitivity on the wavelength modulation is enhanced with increasing wavelengths. The telecommunication wavelength range was then utilized to detect Mycobacterium tuberculosis (MTB) deoxyribonucleic acid (DNA) under two situations, without immobilization and with 5'-thiol end labeled IS6100 DNA probes, for SPR sensitivity comparison. The experimental data demonstrated that the SPR sensitivity increased more than 13 times with the wavelength modulation after immobilization. Since the operating wavelength accuracy of a tunable laser source can be controlled within 0.001 nm, the sensitivity and resolution on immobilized MTB DNA were determined as 1.04 nm/(μg/mL) and 0.9 ng/mL, respectively. View Full-Text
Keywords: Mycobacterium tuberculosis; surface plasmon resonance; immobilization; wavelength modulation Mycobacterium tuberculosis; surface plasmon resonance; immobilization; wavelength modulation
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Hsu, S.-H.; Hung, S.-C.; Chen, Y.-K.; Jian, Z.-H. Surface Plasmon Resonator Using High Sensitive Resonance Telecommunication Wavelengths for DNA Sensors of Mycobacterium Tuberculosis with Thiol-Modified Probes. Sensors 2015, 15, 331-340.

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