Special Issue "Next-Generation Nucleic Acid Sensors"
Deadline for manuscript submissions: 28 February 2015
Dr. Matteo Castronovo
1. Adjunct Assistant Professor, Department of Biology, Temple University, Philadelphia, PA, USA
2. Department of Medical and Biological Sciences, University of Udine, Piazzale Kolbe, 4, 33100 Udine, Italy
Phone: +39 0432 494360
Interests: atomic force microscopy; crowding; denaturation; detection; DNA; electrochemistry; enzymes; fluorescence; hybridization; molecular device; nanoarray; nanomanipulation; nanomedicine; nanotechnology; nucleic acids; nucleases; RNA; self-assembled monolayers; self-assembly; steric hindrance; surfaces
The explosive development of nucleic acid (NA) nanotechnologies has underscored the importance of the inherent capacity of NAs to self-associate, thus providing an essentially limitless wellspring for the directed formation of complex assemblies with diverse functions.
DNA, RNA, and related NA polymers provide the building blocks for the bottoms-up fabrication of sensing devices. In addition, the high-throughput detection/analysis of NAs also requires the confinement of functional probes within highly dense microchips, which involves the top-down fabrication of microfluidic and electronic components. The development of next-generation of NA sensors will require functional integration between the bottom-up and top-down approaches, thus necessitating strongly interdisciplinary research approaches. This special issue aims to bring together, under one cover, innovative bottom-up and top-down approaches for NA detection and analysis.
Papers addressing a wide range of bottoms-up, top-down, and hybrid approaches for the quantitative detection or analysis of DNA and RNA molecules are sought; topics include, but are not necessarily limited to, recent developments in the following areas: the manipulation of long DNA or RNA molecules through single-molecule microscopy and spectroscopic approaches; biological and solid-state nanopores; microfluidic systems; DNA or RNA micro- and nano-arrays; DNA sequencing; solid-state PCR; digital PCR; DNA- or RNA-functionalized nanoparticles; and DNA origami.
Both review articles and original research papers relating to the development or the application of advanced technologies for sensing nucleic acids, as well as nanoscience studies relating to the novel behaviors of NA-based, self-assembled nanodevices are sought.
Dr. Matteo Castronovo
Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. Papers will be published continuously (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.
Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are refereed through a peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Sensors is an international peer-reviewed Open Access monthly journal published by MDPI.
- DNA nanosensors
- lab-on-a-chip DNA sensors
- functional DNA nanotechnologies
- DNA self-assembly
- DNA nanoarrays
- next-generation sequencing
- single molecule DNA/RNA detection
The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.
Type of Paper: Article
Title: mRNA-Responsive G-quadruplex-Based Drug Release System
Authors: Hidenobu Yaku, Takeshi Murashima, Naoki Sugimoto, Daisuke Miyoshi
Affiliation: Faculty of Frontiers of Innovative Reseach in Science and Technology (FIRST), Konan University, Japan
Abstract: G-quadruplex-based drug delivery carriers (GDDCs) that can release a G-quadruplex-ligand as a telomerase inhibitor in response to EGFR mRNA were designed. A loop sequence of the GDDCs is complementary with EGFR mRNA so that a hybridization between them may unfold the G-quadruplex, resulting in a release of the ligand. Circular dichroism (CD) analysis showed that the GDDCs formed
(3+1) G-quadruplex in 100 mM K+ and 10 mM Mg2+. Visible absorbance analysis showed that the GDDCs bound to copper(II) anionic phthalocyanine (Cu-APC), a specific G-quadruplex-ligand over duplex. Furthermore, an addition of an oligo RNA corresponding to EGFR mRNA released Cu-APC from the GDDC with a 30mer length loop by unfolding the G-quadruplex structure.
Last update: 3 September 2014