Engineering Genetically Encoded FRET Sensors
AbstractFörster Resonance Energy Transfer (FRET) between two fluorescent proteins can be exploited to create fully genetically encoded and thus subcellularly targetable sensors. FRET sensors report changes in energy transfer between a donor and an acceptor fluorescent protein that occur when an attached sensor domain undergoes a change in conformation in response to ligand binding. The design of sensitive FRET sensors remains challenging as there are few generally applicable design rules and each sensor must be optimized anew. In this review we discuss various strategies that address this shortcoming, including rational design approaches that exploit self-associating fluorescent domains and the directed evolution of FRET sensors using high-throughput screening.
Scifeed alert for new publicationsNever miss any articles matching your research from any publisher
- Get alerts for new papers matching your research
- Find out the new papers from selected authors
- Updated daily for 49'000+ journals and 6000+ publishers
- Define your Scifeed now
Lindenburg, L.; Merkx, M. Engineering Genetically Encoded FRET Sensors. Sensors 2014, 14, 11691-11713.
Lindenburg L, Merkx M. Engineering Genetically Encoded FRET Sensors. Sensors. 2014; 14(7):11691-11713.Chicago/Turabian Style
Lindenburg, Laurens; Merkx, Maarten. 2014. "Engineering Genetically Encoded FRET Sensors." Sensors 14, no. 7: 11691-11713.