Abstract: Proteobacteria use quorum sensing to regulate target gene expression in response to population density. Quorum sensing (QS) is achieved via so-called signalling molecules and the best-studied QS signalling system uses N-acyl homoserine lactones (AHLs). This study aimed to identify and characterize the production of AHLs by a bacterium ND03 isolated from a Malaysian tropical rainforest waterfall. Molecular identification showed that ND03 is a Pantoea sp. closely related to Pantoea rodasii. We used Chromobacterium violaceum CV026, an AHL biosensor for preliminary AHL production screening and then used high resolution triple quadrupole liquid chromatography-mass spectrometry, to confirm that P. rodasii strain ND03 produced N-(3-oxo-hexanoyl)-L-homoserine lactone (3-oxo-C6-HSL). To the best of our knowledge, this is the first report for such a discovery in P. rodasii strain ND03.
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Yunos, N.Y.M.; Tan, W.-S.; Mohamad, N.I.; Tan, P.-W.; Adrian, T.-G.-S.; Yin, W.-F.; Chan, K.-G. Discovery of Pantoea rodasii Strain ND03 that Produces N-(3-Oxo-hexanoyl)-L-homoserine Lactone. Sensors 2014, 14, 9145-9152.
Yunos NYM, Tan W-S, Mohamad NI, Tan P-W, Adrian T-G-S, Yin W-F, Chan K-G. Discovery of Pantoea rodasii Strain ND03 that Produces N-(3-Oxo-hexanoyl)-L-homoserine Lactone. Sensors. 2014; 14(5):9145-9152.
Yunos, Nina Y.M.; Tan, Wen-Si; Mohamad, Nur I.; Tan, Pui-Wan; Adrian, Tan-Guan-Sheng; Yin, Wai-Fong; Chan, Kok-Gan. 2014. "Discovery of Pantoea rodasii Strain ND03 that Produces N-(3-Oxo-hexanoyl)-L-homoserine Lactone." Sensors 14, no. 5: 9145-9152.