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Sensors 2014, 14(5), 9145-9152; doi:10.3390/s140509145
Article

Discovery of Pantoea rodasii Strain ND03 that Produces N-(3-Oxo-hexanoyl)-L-homoserine Lactone

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Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia
* Author to whom correspondence should be addressed.
Received: 8 April 2014 / Revised: 13 May 2014 / Accepted: 16 May 2014 / Published: 23 May 2014
(This article belongs to the Section Biosensors)
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Abstract

Proteobacteria use quorum sensing to regulate target gene expression in response to population density. Quorum sensing (QS) is achieved via so-called signalling molecules and the best-studied QS signalling system uses N-acyl homoserine lactones (AHLs). This study aimed to identify and characterize the production of AHLs by a bacterium ND03 isolated from a Malaysian tropical rainforest waterfall. Molecular identification showed that ND03 is a Pantoea sp. closely related to Pantoea rodasii. We used Chromobacterium violaceum CV026, an AHL biosensor for preliminary AHL production screening and then used high resolution triple quadrupole liquid chromatography-mass spectrometry, to confirm that P. rodasii strain ND03 produced N-(3-oxo-hexanoyl)-L-homoserine lactone (3-oxo-C6-HSL). To the best of our knowledge, this is the first report for such a discovery in P. rodasii strain ND03.
Keywords: cell-to-cell communication; mass spectrometry; triple quadrupole liquid chromatography mass spectrometry; quorum sensing; N-(3-oxohexanoyl) homoserine-lactone (3-oxo-C6-HSL); Pantoea rodasii cell-to-cell communication; mass spectrometry; triple quadrupole liquid chromatography mass spectrometry; quorum sensing; N-(3-oxohexanoyl) homoserine-lactone (3-oxo-C6-HSL); Pantoea rodasii
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Yunos, N.Y.M.; Tan, W.-S.; Mohamad, N.I.; Tan, P.-W.; Adrian, T.-G.-S.; Yin, W.-F.; Chan, K.-G. Discovery of Pantoea rodasii Strain ND03 that Produces N-(3-Oxo-hexanoyl)-L-homoserine Lactone. Sensors 2014, 14, 9145-9152.

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