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Sensors 2014, 14(5), 9145-9152; doi:10.3390/s140509145
Article

Discovery of Pantoea rodasii Strain ND03 that Produces N-(3-Oxo-hexanoyl)-L-homoserine Lactone

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Received: 8 April 2014 / Revised: 13 May 2014 / Accepted: 16 May 2014 / Published: 23 May 2014
(This article belongs to the Section Biosensors)
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Abstract

Proteobacteria use quorum sensing to regulate target gene expression in response to population density. Quorum sensing (QS) is achieved via so-called signalling molecules and the best-studied QS signalling system uses N-acyl homoserine lactones (AHLs). This study aimed to identify and characterize the production of AHLs by a bacterium ND03 isolated from a Malaysian tropical rainforest waterfall. Molecular identification showed that ND03 is a Pantoea sp. closely related to Pantoea rodasii. We used Chromobacterium violaceum CV026, an AHL biosensor for preliminary AHL production screening and then used high resolution triple quadrupole liquid chromatography-mass spectrometry, to confirm that P. rodasii strain ND03 produced N-(3-oxo-hexanoyl)-L-homoserine lactone (3-oxo-C6-HSL). To the best of our knowledge, this is the first report for such a discovery in P. rodasii strain ND03.
Keywords: cell-to-cell communication; mass spectrometry; triple quadrupole liquid chromatography mass spectrometry; quorum sensing; N-(3-oxohexanoyl) homoserine-lactone (3-oxo-C6-HSL); Pantoea rodasii cell-to-cell communication; mass spectrometry; triple quadrupole liquid chromatography mass spectrometry; quorum sensing; N-(3-oxohexanoyl) homoserine-lactone (3-oxo-C6-HSL); Pantoea rodasii
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Yunos, N.Y.M.; Tan, W.-S.; Mohamad, N.I.; Tan, P.-W.; Adrian, T.-G.-S.; Yin, W.-F.; Chan, K.-G. Discovery of Pantoea rodasii Strain ND03 that Produces N-(3-Oxo-hexanoyl)-L-homoserine Lactone. Sensors 2014, 14, 9145-9152.

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