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Sensors 2014, 14(5), 9132-9144; doi:10.3390/s140509132

A Compact Immunoassay Platform Based on a Multicapillary Glass Plate

*  and
Department of Applied Chemistry, Graduate School of Urban Environmental Sciences, Tokyo Metropolitan University, 1-1 Minamiohsawa, Hachioji, Tokyo 192-0397, Japan
* Author to whom correspondence should be addressed.
Received: 15 April 2014 / Revised: 15 May 2014 / Accepted: 20 May 2014 / Published: 23 May 2014
(This article belongs to the Section Biosensors)
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A highly sensitive, rapid immunoassay performed in the multi-channels of a micro-well array consisting of a multicapillary glass plate (MCP) and a polydimethylsiloxane (PDMS) slide is described. The micro-dimensions and large surface area of the MCP permitted the diffusion distance to be decreased and the reaction efficiency to be increased. To confirm the concept of the method, human immunoglobulin A (h-IgA) was measured using both the proposed immunoassay system and the traditional 96-well plate method. The proposed method resulted in a 1/5-fold decrease of immunoassay time, and a 1/56-fold cut in reagent consumption with a 0.05 ng/mL of limit of detection (LOD) for IgA. The method was also applied to saliva samples obtained from healthy volunteers. The results correlated well to those obtained by the 96-well plate method. The method has the potential for use in disease diagnostic or on-site immunoassays.
Keywords: multicapillary glass plate; compact immunoassay; enzyme-linked immunosorbent assay multicapillary glass plate; compact immunoassay; enzyme-linked immunosorbent assay
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Xue, S.; Zeng, H.; Yang, J.; Nakajima, H.; Uchiyama, K. A Compact Immunoassay Platform Based on a Multicapillary Glass Plate. Sensors 2014, 14, 9132-9144.

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