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Sensors 2014, 14(5), 9117-9131; doi:10.3390/s140509117
Review

Point-of-care Diagnostic Tools to Detect Circulating MicroRNAS as Biomarkers of Disease

Received: 26 February 2014; in revised form: 30 April 2014 / Accepted: 4 May 2014 / Published: 22 May 2014
(This article belongs to the Section Biosensors)
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Abstract: MicroRNAs or miRNAs are a form of small non-coding RNAs (ncRNAs) of 19–22 nucleotides in length in their mature form. miRNAs are transcribed in the nucleus of all cells from large precursors, many of which have several kilobases in length. Originally identified as intracellular modulators of protein synthesis via posttranscriptional gene silencing, more recently it has been found that miRNAs can travel in extracellular human fluids inside specialized vesicles known as exosomes. We will be referring to this miRNAs as circulating microRNAs. More interestingly, the miRNA content inside exosomes changes during pathological events. In the present review we analyze the literature about circulating miRNAs and their possible use as biomarkers. Furthermore, we explore their future in point-of-care (POC) diagnostics and provide an example of a portable POC apparatus useful in the detection of circulating miRNAs.
Keywords: microRNAs; exosomes; fluids; polymerase chain reaction (PCR); microarrays; diagnostics microRNAs; exosomes; fluids; polymerase chain reaction (PCR); microarrays; diagnostics
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Vaca, L. Point-of-care Diagnostic Tools to Detect Circulating MicroRNAS as Biomarkers of Disease. Sensors 2014, 14, 9117-9131.

AMA Style

Vaca L. Point-of-care Diagnostic Tools to Detect Circulating MicroRNAS as Biomarkers of Disease. Sensors. 2014; 14(5):9117-9131.

Chicago/Turabian Style

Vaca, Luis. 2014. "Point-of-care Diagnostic Tools to Detect Circulating MicroRNAS as Biomarkers of Disease." Sensors 14, no. 5: 9117-9131.



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