Nanomechanical DNA Origami pH Sensors
AbstractSingle-molecule pH sensors have been developed by utilizing molecular imaging of pH-responsive shape transition of nanomechanical DNA origami devices with atomic force microscopy (AFM). Short DNA fragments that can form i-motifs were introduced to nanomechanical DNA origami devices with pliers-like shape (DNA Origami Pliers), which consist of two levers of 170-nm long and 20-nm wide connected at a Holliday-junction fulcrum. DNA Origami Pliers can be observed as in three distinct forms; cross, antiparallel and parallel forms, and cross form is the dominant species when no additional interaction is introduced to DNA Origami Pliers. Introduction of nine pairs of 12-mer sequence (5'-AACCCCAACCCC-3'), which dimerize into i-motif quadruplexes upon protonation of cytosine, drives transition of DNA Origami Pliers from open cross form into closed parallel form under acidic conditions. Such pH-dependent transition was clearly imaged on mica in molecular resolution by AFM, showing potential application of the system to single-molecular pH sensors. View Full-Text
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Kuzuya, A.; Watanabe, R.; Yamanaka, Y.; Tamaki, T.; Kaino, M.; Ohya, Y. Nanomechanical DNA Origami pH Sensors. Sensors 2014, 14, 19329-19335.
Kuzuya A, Watanabe R, Yamanaka Y, Tamaki T, Kaino M, Ohya Y. Nanomechanical DNA Origami pH Sensors. Sensors. 2014; 14(10):19329-19335.Chicago/Turabian Style
Kuzuya, Akinori; Watanabe, Ryosuke; Yamanaka, Yusei; Tamaki, Takuya; Kaino, Masafumi; Ohya, Yuichi. 2014. "Nanomechanical DNA Origami pH Sensors." Sensors 14, no. 10: 19329-19335.