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Development of an Enzyme-Linked Immunosorbent Assay for Dibutyl Phthalate in Liquor
State Key Laboratory of Food Science & Technology, School of Food Science & Technology, Jiangnan University, Wuxi 214122, China
Research Centre of Hunan Entry–Exit Inspection and Quarantine Bureau, Changsha 410001, China
* Author to whom correspondence should be addressed.
Received: 24 April 2013; in revised form: 8 June 2013 / Accepted: 20 June 2013 / Published: 27 June 2013
Abstract: A monoclonal antibody specifically recognizing dibutyl phthalate (DBP) was prepared based on a hapten (di-n-butyl-4-aminophthalate). After optimizing various parameters such as concentrations of antibody, coating antigen and composition of the assay buffer, an inhibition curve was plotted with the 50% inhibition concentration value (IC50) 33.6 ± 2.5 ng/mL. A low level of cross-reactivity (<5%) was found for other phthalate esters. Recovery tests were conducted using liquor simulant (a mixture of water and ethanol) at two fortification levels (100 ng/mL and 300 ng/mL). The recovery rates ranged from 84.7% to 94.5% with a coefficient of variation between 7.1% and 12.8%. Nine liquor samples of different alcoholic strengths were detected using the proposed measure and confirmatory analysis was performed using liquid chromatography-mass spectroscopy (LC-MS). The detection results showed good consistency between the two measures and all the data above indicated that the proposed ELISA could be applied in DBP screening.
Keywords: di-n-butyl phthalate; ELISA; liquor; monoclonal antibody
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MDPI and ACS Style
Kuang, H.; Liu, L.; Xu, L.; Ma, W.; Guo, L.; Wang, L.; Xu, C. Development of an Enzyme-Linked Immunosorbent Assay for Dibutyl Phthalate in Liquor. Sensors 2013, 13, 8331-8339.
Kuang H, Liu L, Xu L, Ma W, Guo L, Wang L, Xu C. Development of an Enzyme-Linked Immunosorbent Assay for Dibutyl Phthalate in Liquor. Sensors. 2013; 13(7):8331-8339.
Kuang, Hua; Liu, Liqiang; Xu, Liguang; Ma, Wei; Guo, Lingling; Wang, Libing; Xu, Chuanlai. 2013. "Development of an Enzyme-Linked Immunosorbent Assay for Dibutyl Phthalate in Liquor." Sensors 13, no. 7: 8331-8339.