Next Article in Journal
Organic Plasmon-Emitting Diodes for Detecting Refractive Index Variation
Previous Article in Journal
Energy Consumption and Control Response Evaluations of AODV Routing in WSANs for Building-Temperature Control
Sensors 2013, 13(7), 8331-8339; doi:10.3390/s130708331
Article

Development of an Enzyme-Linked Immunosorbent Assay for Dibutyl Phthalate in Liquor

1,* , 1
,
1
,
1
,
1
,
2
 and
1
1 State Key Laboratory of Food Science & Technology, School of Food Science & Technology, Jiangnan University, Wuxi 214122, China 2 Research Centre of Hunan Entry–Exit Inspection and Quarantine Bureau, Changsha 410001, China
* Author to whom correspondence should be addressed.
Received: 24 April 2013 / Revised: 8 June 2013 / Accepted: 20 June 2013 / Published: 27 June 2013
(This article belongs to the Section Biosensors)
View Full-Text   |   Download PDF [167 KB, uploaded 21 June 2014]   |   Browse Figures
SciFeed

Abstract

A monoclonal antibody specifically recognizing dibutyl phthalate (DBP) was prepared based on a hapten (di-n-butyl-4-aminophthalate). After optimizing various parameters such as concentrations of antibody, coating antigen and composition of the assay buffer, an inhibition curve was plotted with the 50% inhibition concentration value (IC50) 33.6 ± 2.5 ng/mL. A low level of cross-reactivity (<5%) was found for other phthalate esters. Recovery tests were conducted using liquor simulant (a mixture of water and ethanol) at two fortification levels (100 ng/mL and 300 ng/mL). The recovery rates ranged from 84.7% to 94.5% with a coefficient of variation between 7.1% and 12.8%. Nine liquor samples of different alcoholic strengths were detected using the proposed measure and confirmatory analysis was performed using liquid chromatography-mass spectroscopy (LC-MS). The detection results showed good consistency between the two measures and all the data above indicated that the proposed ELISA could be applied in DBP screening.
Keywords: di-n-butyl phthalate; ELISA; liquor; monoclonal antibody di-n-butyl phthalate; ELISA; liquor; monoclonal antibody
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote |
RIS
MDPI and ACS Style

Kuang, H.; Liu, L.; Xu, L.; Ma, W.; Guo, L.; Wang, L.; Xu, C. Development of an Enzyme-Linked Immunosorbent Assay for Dibutyl Phthalate in Liquor. Sensors 2013, 13, 8331-8339.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert