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Sensors 2013, 13(7), 8331-8339; doi:10.3390/s130708331

Development of an Enzyme-Linked Immunosorbent Assay for Dibutyl Phthalate in Liquor

1,* , 1
1 State Key Laboratory of Food Science & Technology, School of Food Science & Technology, Jiangnan University, Wuxi 214122, China 2 Research Centre of Hunan Entry–Exit Inspection and Quarantine Bureau, Changsha 410001, China
* Author to whom correspondence should be addressed.
Received: 24 April 2013 / Revised: 8 June 2013 / Accepted: 20 June 2013 / Published: 27 June 2013
(This article belongs to the Section Biosensors)
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A monoclonal antibody specifically recognizing dibutyl phthalate (DBP) was prepared based on a hapten (di-n-butyl-4-aminophthalate). After optimizing various parameters such as concentrations of antibody, coating antigen and composition of the assay buffer, an inhibition curve was plotted with the 50% inhibition concentration value (IC50) 33.6 ± 2.5 ng/mL. A low level of cross-reactivity (<5%) was found for other phthalate esters. Recovery tests were conducted using liquor simulant (a mixture of water and ethanol) at two fortification levels (100 ng/mL and 300 ng/mL). The recovery rates ranged from 84.7% to 94.5% with a coefficient of variation between 7.1% and 12.8%. Nine liquor samples of different alcoholic strengths were detected using the proposed measure and confirmatory analysis was performed using liquid chromatography-mass spectroscopy (LC-MS). The detection results showed good consistency between the two measures and all the data above indicated that the proposed ELISA could be applied in DBP screening.
Keywords: di-n-butyl phthalate; ELISA; liquor; monoclonal antibody di-n-butyl phthalate; ELISA; liquor; monoclonal antibody
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Kuang, H.; Liu, L.; Xu, L.; Ma, W.; Guo, L.; Wang, L.; Xu, C. Development of an Enzyme-Linked Immunosorbent Assay for Dibutyl Phthalate in Liquor. Sensors 2013, 13, 8331-8339.

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