Next Article in Journal
Next Article in Special Issue
Previous Article in Journal
Previous Article in Special Issue
Sensors 2013, 13(11), 15348-15363; doi:10.3390/s131115348
Article

Label-Enhanced Surface Plasmon Resonance: A New Concept for Improved Performance in Optical Biosensor Analysis

1,2,* , 3
, 3
, 2
 and 1
Received: 20 September 2013; in revised form: 29 October 2013 / Accepted: 2 November 2013 / Published: 8 November 2013
(This article belongs to the Special Issue Plasmonics and Nanoplasmonics Biosensors)
View Full-Text   |   Download PDF [461 KB, uploaded 21 June 2014]   |   Browse Figures
Abstract: Surface plasmon resonance (SPR) is a well-established optical biosensor technology with many proven applications in the study of molecular interactions as well as in surface and material science. SPR is usually applied in the label-free mode which may be advantageous in cases where the presence of a label may potentially interfere with the studied interactions per se. However, the fundamental challenges of label-free SPR in terms of limited sensitivity and specificity are well known. Here we present a new concept called label-enhanced SPR, which is based on utilizing strongly absorbing dye molecules in combination with the evaluation of the full shape of the SPR curve, whereby the sensitivity as well as the specificity of SPR is significantly improved. The performance of the new label-enhanced SPR method was demonstrated by two simple model assays: a small molecule assay and a DNA hybridization assay. The small molecule assay was used to demonstrate the sensitivity enhancement of the method, and how competitive assays can be used for relative affinity determination. The DNA assay was used to demonstrate the selectivity of the assay, and the capabilities in eliminating noise from bulk liquid composition variations.
Keywords: biosensor; surface plasmon resonance; SPR; label; sensitivity; specificity biosensor; surface plasmon resonance; SPR; label; sensitivity; specificity
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Granqvist, N.; Hanning, A.; Eng, L.; Tuppurainen, J.; Viitala, T. Label-Enhanced Surface Plasmon Resonance: A New Concept for Improved Performance in Optical Biosensor Analysis. Sensors 2013, 13, 15348-15363.

AMA Style

Granqvist N, Hanning A, Eng L, Tuppurainen J, Viitala T. Label-Enhanced Surface Plasmon Resonance: A New Concept for Improved Performance in Optical Biosensor Analysis. Sensors. 2013; 13(11):15348-15363.

Chicago/Turabian Style

Granqvist, Niko; Hanning, Anders; Eng, Lars; Tuppurainen, Jussi; Viitala, Tapani. 2013. "Label-Enhanced Surface Plasmon Resonance: A New Concept for Improved Performance in Optical Biosensor Analysis." Sensors 13, no. 11: 15348-15363.



Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert