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ABO Blood-Typing Using an Antibody Array Technique Based on Surface Plasmon Resonance Imaging
Materials Science and Engineering Programme, Faculty of Science, Mahidol University, Rama 6 Rd., Phayathai, Rajathavee, Bangkok 10400, Thailand
Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University Rama 6 Rd., Phayathai, Rajathavee, Bangkok 10400, Thailand
School of Electronic Engineering and School of Telecommunications Engineering, Suranaree University of Technology, 111 University Avenue, Muang District, Nakhon Ratchasima 30000, Thailand
Photonics Technology Laboratory, National Electronics and Computer Technology Center (NECTEC), Pathumthani 12120, Thailand
Physics Department, Faculty of Science, Mahidol University, Rama 6 Rd., Phayathai, Rajathavee, Bangkok 10400, Thailand
* Author to whom correspondence should be addressed.
Received: 4 July 2013; in revised form: 23 August 2013 / Accepted: 2 September 2013 / Published: 9 September 2013
Abstract: In this study, readily available antibodies that are used in standard agglutination tests were evaluated for their use in ABO blood typing by a surface plasmon resonance imaging (SPR imaging) technique. Five groups of antibodies, including mixed clones of anti-A, anti-B, and anti-AB, and single clones of anti-A and anti-B, were used to construct the five-line detection arrays using a multichannel flow cell in the SPR imager. The red blood cell (RBC) samples were applied to a multichannel flow cell that was orthogonal to the detection line arrays for blood group typing. We found that the blood samples were correctly grouped in less than 12 min by the SPR imaging technique, and the results were consistent with those of the standard agglutination technique for all 60 samples. We found that mixed clones of antibodies provided 33%–68% greater change in the SPR signal than the single-clone antibodies. Applying the SPR imaging technique using readily available antibodies may reduce the costs of the antibodies, shorten the measurement time, and increase the throughput.
Keywords: ABO blood typing; surface plasmon resonance imaging (SPR imaging); immunoassay; antibody arrays
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Houngkamhang, N.; Vongsakulyanon, A.; Peungthum, P.; Sudprasert, K.; Kitpoka, P.; Kunakorn, M.; Sutapun, B.; Amarit, R.; Somboonkaew, A.; Srikhirin, T. ABO Blood-Typing Using an Antibody Array Technique Based on Surface Plasmon Resonance Imaging. Sensors 2013, 13, 11913-11922.
Houngkamhang N, Vongsakulyanon A, Peungthum P, Sudprasert K, Kitpoka P, Kunakorn M, Sutapun B, Amarit R, Somboonkaew A, Srikhirin T. ABO Blood-Typing Using an Antibody Array Technique Based on Surface Plasmon Resonance Imaging. Sensors. 2013; 13(9):11913-11922.
Houngkamhang, Nongluck; Vongsakulyanon, Apirom; Peungthum, Patjaree; Sudprasert, Krisda; Kitpoka, Pimpun; Kunakorn, Mongkol; Sutapun, Boonsong; Amarit, Ratthasart; Somboonkaew, Armote; Srikhirin, Toemsak. 2013. "ABO Blood-Typing Using an Antibody Array Technique Based on Surface Plasmon Resonance Imaging." Sensors 13, no. 9: 11913-11922.