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Sensors 2012, 12(9), 11684-11696; doi:10.3390/s120911684

A Fast and Sensitive Quantitative Lateral Flow Immunoassay for Cry1Ab Based on a Novel Signal Amplification Conjugate

1 Department of Control Science and Engineering, Zhejiang University, Hangzhou 310058, China 2 Department of Biosytems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China
* Author to whom correspondence should be addressed.
Received: 5 July 2012 / Revised: 6 August 2012 / Accepted: 20 August 2012 / Published: 27 August 2012
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A novel lateral flow immunoassay (LFIA) signal amplification strategy for the detection of Cry1Ab based on amplification via a polylysine (PL) chain and biotin-streptavidin system (BSAS) is described. In this system, multiple fluorescence dyes (FL) were directly coated on the surface of PL and conjugated with antibody via the BSAS for construction of novel signal amplification (FLPL-BSAS-mAb1) conjugates, in which FL, PL and BSAS were employed to improve the sensitivity of LFIA. Compared with conventional LFIA, the sensitivity of FLPL-BSAS-mAb1-based LFIA was increased by approximately 100-fold. Quantified linearity was achieved in the value range of 0–1,000 pg/mL. The limit of detection (LOD) was reached 10 pg/mL after optimization of reaction conditions. To our knowledge, this represents one of the most sensitive LFIA for Cry1Ab yet reported. Furthermore, the detection time for this method was about 10 min. Therefore, it should be an attractive alternative compared to conventional immunoassays in routine control for Cry1Ab.
Keywords: cry1Ab; fluorescence dye; lateral flow biosensor; polylysine cry1Ab; fluorescence dye; lateral flow biosensor; polylysine
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Chen, C.; Wu, J. A Fast and Sensitive Quantitative Lateral Flow Immunoassay for Cry1Ab Based on a Novel Signal Amplification Conjugate. Sensors 2012, 12, 11684-11696.

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