Next Article in Journal
Study Designs and Statistical Analyses for Biomarker Research
Next Article in Special Issue
A Low-Cost, Portable, High-Throughput Wireless Sensor System for Phonocardiography Applications
Previous Article in Journal
Enabling Flexible and Continuous Capability Invocation in Mobile Prosumer Environments
Previous Article in Special Issue
A Urinary Bcl-2 Surface Acoustic Wave Biosensor for Early Ovarian Cancer Detection
Sensors 2012, 12(7), 8955-8965; doi:10.3390/s120708955

An Oxidase-Based Electrochemical Fluidic Sensor with High-Sensitivity and Low-Interference by On-Chip Oxygen Manipulation

Received: 28 May 2012 / Revised: 20 June 2012 / Accepted: 21 June 2012 / Published: 29 June 2012
View Full-Text   |   Download PDF [1227 KB, uploaded 21 June 2014]   |   Browse Figures


Utilizing a simple fluidic structure, we demonstrate the improved performance of oxidase-based enzymatic biosensors. Electrolysis of water is utilized to generate bubbles to manipulate the oxygen microenvironment close to the biosensor in a fluidic channel. For the proper enzyme reactions to occur, a simple mechanical procedure of manipulating bubbles was developed to maximize the oxygen level while minimizing the pH change after electrolysis. The sensors show improved sensitivities based on the oxygen dependency of enzyme reaction. In addition, this oxygen-rich operation minimizes the ratio of electrochemical interference signal by ascorbic acid during sensor operation (i.e., amperometric detection of hydrogen peroxide). Although creatinine sensors have been used as the model system in this study, this method is applicable to many other biosensors that can use oxidase enzymes (e.g., glucose, alcohol, phenol, etc.) to implement a viable component for in-line fluidic sensor systems.
Keywords: bubble; electrolysis; oxygen; hydrogen; biosensor; oxidase bubble; electrolysis; oxygen; hydrogen; biosensor; oxidase
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
MDPI and ACS Style

Radhakrishnan, N.; Park, J.; Kim, C.-S. An Oxidase-Based Electrochemical Fluidic Sensor with High-Sensitivity and Low-Interference by On-Chip Oxygen Manipulation. Sensors 2012, 12, 8955-8965.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here


Cited By

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert