Sensors 2012, 12(6), 7423-7437; doi:10.3390/s120607423
Article

A Urinary Bcl-2 Surface Acoustic Wave Biosensor for Early Ovarian Cancer Detection

1 Department of Mechanical Engineering, University of South Florida, 4202 E. Fowler Avenue, ENB 118, Tampa, FL 33620, USA 2 Department of Electrical and Electronics Engineering, Marmara University, Goztepe 34722, Istanbul, Turkey 3 Department of Pathology and Cell Biology, University of South Florida Health, 12901 Bruce B. Downs Blvd., MDC 11, Tampa, FL 33612, USA
* Author to whom correspondence should be addressed.
Received: 31 March 2012; in revised form: 22 May 2012 / Accepted: 28 May 2012 / Published: 31 May 2012
PDF Full-text Download PDF Full-Text [1112 KB, uploaded 31 May 2012 10:44 CEST]
Abstract: In this study, the design, fabrication, surface functionalization and experimental characterization of an ultrasonic MEMS biosensor for urinary anti-apoptotic protein B-cell lymphoma 2 (Bcl-2) detection with sub ng/mL sensitivity is presented. It was previously shown that urinary Bcl-2 levels are reliably elevated during early and late stages of ovarian cancer. Our biosensor uses shear horizontal (SH) surface acoustic waves (SAWs) on surface functionalized ST-cut Quartz to quantify the mass loading change by protein adhesion to the delay path. SH-SAWs were generated and received by a pair of micro-fabricated interdigital transducers (IDTs) separated by a judiciously designed delay path. The delay path was surface-functionalized with monoclonal antibodies, ODMS, Protein A/G and Pluronic F127 for optimal Bcl-2 capture with minimal non-specific adsorption. Bcl-2 concentrations were quantified by the resulting resonance frequency shift detected by a custom designed resonator circuit. The target sensitivity for diagnosis and identifying the stage of ovarian cancer was successfully achieved with demonstrated Bcl-2 detection capability of 500 pg/mL. It was also shown that resonance frequency shift increases linearly with increasing Bcl-2 concentration.
Keywords: ovarian cancer; MEMS; piezoelectric; sensor; early detection; Bcl-2; SH; SAW; SAM; ultrasound

Article Statistics

Load and display the download statistics.

Citations to this Article

Cite This Article

MDPI and ACS Style

Onen, O.; Sisman, A.; Gallant, N.D.; Kruk, P.; Guldiken, R. A Urinary Bcl-2 Surface Acoustic Wave Biosensor for Early Ovarian Cancer Detection. Sensors 2012, 12, 7423-7437.

AMA Style

Onen O, Sisman A, Gallant ND, Kruk P, Guldiken R. A Urinary Bcl-2 Surface Acoustic Wave Biosensor for Early Ovarian Cancer Detection. Sensors. 2012; 12(6):7423-7437.

Chicago/Turabian Style

Onen, Onursal; Sisman, Alper; Gallant, Nathan D.; Kruk, Patricia; Guldiken, Rasim. 2012. "A Urinary Bcl-2 Surface Acoustic Wave Biosensor for Early Ovarian Cancer Detection." Sensors 12, no. 6: 7423-7437.

Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert