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A Urinary Bcl-2 Surface Acoustic Wave Biosensor for Early Ovarian Cancer Detection
AbstractIn this study, the design, fabrication, surface functionalization and experimental characterization of an ultrasonic MEMS biosensor for urinary anti-apoptotic protein B-cell lymphoma 2 (Bcl-2) detection with sub ng/mL sensitivity is presented. It was previously shown that urinary Bcl-2 levels are reliably elevated during early and late stages of ovarian cancer. Our biosensor uses shear horizontal (SH) surface acoustic waves (SAWs) on surface functionalized ST-cut Quartz to quantify the mass loading change by protein adhesion to the delay path. SH-SAWs were generated and received by a pair of micro-fabricated interdigital transducers (IDTs) separated by a judiciously designed delay path. The delay path was surface-functionalized with monoclonal antibodies, ODMS, Protein A/G and Pluronic F127 for optimal Bcl-2 capture with minimal non-specific adsorption. Bcl-2 concentrations were quantified by the resulting resonance frequency shift detected by a custom designed resonator circuit. The target sensitivity for diagnosis and identifying the stage of ovarian cancer was successfully achieved with demonstrated Bcl-2 detection capability of 500 pg/mL. It was also shown that resonance frequency shift increases linearly with increasing Bcl-2 concentration.
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Onen, O.; Sisman, A.; Gallant, N.D.; Kruk, P.; Guldiken, R. A Urinary Bcl-2 Surface Acoustic Wave Biosensor for Early Ovarian Cancer Detection. Sensors 2012, 12, 7423-7437.View more citation formats
Onen O, Sisman A, Gallant ND, Kruk P, Guldiken R. A Urinary Bcl-2 Surface Acoustic Wave Biosensor for Early Ovarian Cancer Detection. Sensors. 2012; 12(6):7423-7437.Chicago/Turabian Style
Onen, Onursal; Sisman, Alper; Gallant, Nathan D.; Kruk, Patricia; Guldiken, Rasim. 2012. "A Urinary Bcl-2 Surface Acoustic Wave Biosensor for Early Ovarian Cancer Detection." Sensors 12, no. 6: 7423-7437.