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Single-Cell Electric Lysis on an Electroosmotic-Driven Microfluidic Chip with Arrays of Microwells
Department of Mechanical Engineering, Advanced Institute of Manufacturing with High-Tech Innovation, National Chung Cheng University, Chia Yi, 62102, Taiwan
Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk, 630090, Russia
Department of Biochemistry and Molecular Biology, National Cheng-Kung University, Tainan, 70101, Taiwan
* Authors to whom correspondence should be addressed.
Received: 16 April 2012; in revised form: 15 May 2012 / Accepted: 21 May 2012 / Published: 25 May 2012
(This article belongs to the Special Issue Biochips
Abstract: Accurate analysis at the single-cell level has become a highly attractive tool for investigating cellular content. An electroosmotic-driven microfluidic chip with arrays of 30-µm-diameter microwells was developed for single-cell electric lysis in the present study. The cellular occupancy in the microwells when the applied voltage was 5 V (82.4%) was slightly higher than that at an applied voltage of 10 V (81.8%). When the applied voltage was increased to 15 V, the cellular occupancy in the microwells dropped to 64.3%. More than 50% of the occupied microwells contain individual cells. The results of electric lysis experiments at the single-cell level indicate that the cells were gradually lysed as the DC voltage of 30 V was applied; the cell was fully lysed after 25 s. Single-cell electric lysis was demonstrated in the proposed microfluidic chip, which is suitable for high-throughput cell lysis.
Keywords: microwell; single-cell; electric lysis; electroosmotic-driven; microfluidics
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MDPI and ACS Style
Jen, C.-P.; Amstislavskaya, T.G.; Liu, Y.-H.; Hsiao, J.-H.; Chen, Y.-H. Single-Cell Electric Lysis on an Electroosmotic-Driven Microfluidic Chip with Arrays of Microwells. Sensors 2012, 12, 6967-6977.
Jen C-P, Amstislavskaya TG, Liu Y-H, Hsiao J-H, Chen Y-H. Single-Cell Electric Lysis on an Electroosmotic-Driven Microfluidic Chip with Arrays of Microwells. Sensors. 2012; 12(6):6967-6977.
Jen, Chun-Ping; Amstislavskaya, Tamara G.; Liu, Ya-Hui; Hsiao, Ju-Hsiu; Chen, Yu-Hung. 2012. "Single-Cell Electric Lysis on an Electroosmotic-Driven Microfluidic Chip with Arrays of Microwells." Sensors 12, no. 6: 6967-6977.