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Sensors 2012, 12(6), 6967-6977; doi:10.3390/s120606967

Single-Cell Electric Lysis on an Electroosmotic-Driven Microfluidic Chip with Arrays of Microwells

1,* , 2
1 Department of Mechanical Engineering, Advanced Institute of Manufacturing with High-Tech Innovation, National Chung Cheng University, Chia Yi, 62102, Taiwan 2 Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk, 630090, Russia 3 Department of Biochemistry and Molecular Biology, National Cheng-Kung University, Tainan, 70101, Taiwan
* Authors to whom correspondence should be addressed.
Received: 16 April 2012 / Revised: 15 May 2012 / Accepted: 21 May 2012 / Published: 25 May 2012
(This article belongs to the Special Issue Biochips)
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Accurate analysis at the single-cell level has become a highly attractive tool for investigating cellular content. An electroosmotic-driven microfluidic chip with arrays of 30-µm-diameter microwells was developed for single-cell electric lysis in the present study. The cellular occupancy in the microwells when the applied voltage was 5 V (82.4%) was slightly higher than that at an applied voltage of 10 V (81.8%). When the applied voltage was increased to 15 V, the cellular occupancy in the microwells dropped to 64.3%. More than 50% of the occupied microwells contain individual cells. The results of electric lysis experiments at the single-cell level indicate that the cells were gradually lysed as the DC voltage of 30 V was applied; the cell was fully lysed after 25 s. Single-cell electric lysis was demonstrated in the proposed microfluidic chip, which is suitable for high-throughput cell lysis.
Keywords: microwell; single-cell; electric lysis; electroosmotic-driven; microfluidics microwell; single-cell; electric lysis; electroosmotic-driven; microfluidics
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Jen, C.-P.; Amstislavskaya, T.G.; Liu, Y.-H.; Hsiao, J.-H.; Chen, Y.-H. Single-Cell Electric Lysis on an Electroosmotic-Driven Microfluidic Chip with Arrays of Microwells. Sensors 2012, 12, 6967-6977.

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