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Sensors 2012, 12(6), 6967-6977; doi:10.3390/s120606967
Article

Single-Cell Electric Lysis on an Electroosmotic-Driven Microfluidic Chip with Arrays of Microwells

1,* , 2
,
1
,
1
 and
3,*
1 Department of Mechanical Engineering, Advanced Institute of Manufacturing with High-Tech Innovation, National Chung Cheng University, Chia Yi, 62102, Taiwan 2 Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk, 630090, Russia 3 Department of Biochemistry and Molecular Biology, National Cheng-Kung University, Tainan, 70101, Taiwan
* Authors to whom correspondence should be addressed.
Received: 16 April 2012 / Revised: 15 May 2012 / Accepted: 21 May 2012 / Published: 25 May 2012
(This article belongs to the Special Issue Biochips)
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Abstract

Accurate analysis at the single-cell level has become a highly attractive tool for investigating cellular content. An electroosmotic-driven microfluidic chip with arrays of 30-µm-diameter microwells was developed for single-cell electric lysis in the present study. The cellular occupancy in the microwells when the applied voltage was 5 V (82.4%) was slightly higher than that at an applied voltage of 10 V (81.8%). When the applied voltage was increased to 15 V, the cellular occupancy in the microwells dropped to 64.3%. More than 50% of the occupied microwells contain individual cells. The results of electric lysis experiments at the single-cell level indicate that the cells were gradually lysed as the DC voltage of 30 V was applied; the cell was fully lysed after 25 s. Single-cell electric lysis was demonstrated in the proposed microfluidic chip, which is suitable for high-throughput cell lysis.
Keywords: microwell; single-cell; electric lysis; electroosmotic-driven; microfluidics microwell; single-cell; electric lysis; electroosmotic-driven; microfluidics
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Jen, C.-P.; Amstislavskaya, T.G.; Liu, Y.-H.; Hsiao, J.-H.; Chen, Y.-H. Single-Cell Electric Lysis on an Electroosmotic-Driven Microfluidic Chip with Arrays of Microwells. Sensors 2012, 12, 6967-6977.

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