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A Bioanalytical Platform for Simultaneous Detection and Quantification of Biological Toxins
Sensors 2012, 12(5), 5978-5985; doi:10.3390/s120505978

TNF as Biomarker for Rapid Quantification of Active Staphylococcus Enterotoxin A in Food

*  and
Western Regional Research Center, Agricultural Research Service, US Department of Agriculture, Albany, CA 94710, USA
* Author to whom correspondence should be addressed.
Received: 3 March 2012 / Revised: 19 April 2012 / Accepted: 3 May 2012 / Published: 10 May 2012
(This article belongs to the Special Issue Bioassays)
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Staphylococcus aureus is a major bacterial pathogen which causes clinical infections and food poisoning. This bacterium produces a group of twenty-one enterotoxins (SEs). These enterotoxins have two separate but related biological activities. They cause gastroenteritis and function as superantigens that activate large numbers of T cells. The current method for detection of enterotoxins activity is an in vivo monkey or kitten bioassay; however, this method is not practical to test on a large number of samples. Several immunological assays have been developed however, but these assays cannot distinguish between active toxin which causes food poisoning and inactive toxin, which can bind antibody, but shows no toxicity. The current study demonstrates that short term ex vivo exposure of primary naïve CD4+ T-cells or splenocytes to SEA induces differential expression and secretion of tumor necrosis factor (TNF) protein. We used immunomagnetic beads coated with anti-SEA antibody to specifically isolate SEA from food. After the eluted toxin was added to the cells SEA biological activity was measured by quantifying TNF protein expression or secretion.
Keywords: enterotoxin; food poisoning; TNF; immunomagnetic beads enterotoxin; food poisoning; TNF; immunomagnetic beads
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Rasooly, R.; Hernlem, B. TNF as Biomarker for Rapid Quantification of Active Staphylococcus Enterotoxin A in Food. Sensors 2012, 12, 5978-5985.

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