Sensors 2012, 12(7), 9181-9209; doi:10.3390/s120709181
Review

A Unifying Review of Bioassay-Guided Fractionation, Effect-Directed Analysis and Related Techniques

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Received: 16 May 2012; in revised form: 26 June 2012 / Accepted: 2 July 2012 / Published: 4 July 2012
(This article belongs to the Special Issue Bioassays)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: The success of modern methods in analytical chemistry sometimes obscures the problem that the ever increasing amount of analytical data does not necessarily give more insight of practical relevance. As alternative approaches, toxicity- and bioactivity-based assays can deliver valuable information about biological effects of complex materials in humans, other species or even ecosystems. However, the observed effects often cannot be clearly assigned to specific chemical compounds. In these cases, the establishment of an unambiguous cause-effect relationship is not possible. Effect-directed analysis tries to interconnect instrumental analytical techniques with a biological/biochemical entity, which identifies or isolates substances of biological relevance. Successful application has been demonstrated in many fields, either as proof-of-principle studies or even for complex samples. This review discusses the different approaches, advantages and limitations and finally shows some practical examples. The broad emergence of effect-directed analytical concepts might lead to a true paradigm shift in analytical chemistry, away from ever growing lists of chemical compounds. The connection of biological effects with the identification and quantification of molecular entities leads to relevant answers to many real life questions.
Keywords: toxicity; drug discovery; natural products; environmental analysis; lead identification
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MDPI and ACS Style

Weller, M.G. A Unifying Review of Bioassay-Guided Fractionation, Effect-Directed Analysis and Related Techniques. Sensors 2012, 12, 9181-9209.

AMA Style

Weller MG. A Unifying Review of Bioassay-Guided Fractionation, Effect-Directed Analysis and Related Techniques. Sensors. 2012; 12(7):9181-9209.

Chicago/Turabian Style

Weller, Michael G. 2012. "A Unifying Review of Bioassay-Guided Fractionation, Effect-Directed Analysis and Related Techniques." Sensors 12, no. 7: 9181-9209.

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