Fusion-Triggered Switching of Enzymatic Activity on an Artificial Cell Membrane
AbstractA nanosensory membrane device was constructed for detecting liposome fusion through changes in an enzymatic activity. Inspired by a biological signal transduction system, the device design involved functionalized liposomal membranes prepared by self-assembly of the following molecular components: a synthetic peptide lipid and a phospholipid as matrix membrane components, a Schiff’s base of pyridoxal 5’-phosphate with phosphatidylethanolamine as a thermo-responsive artificial receptor, NADH-dependent L-lactate dehydrogenase as a signal amplifier, and Cu2+ ion as a signal mediator between the receptor and enzyme. The enzymatic activity of the membrane device was adjustable by changing the matrix lipid composition, reflecting the thermotropic phase transition behavior of the lipid membranes, which in turn controlled receptor binding affinity toward the enzyme-inhibiting mediator species. When an effective fusogen anionic polymer was added to these cationic liposomes, membrane fusion occurred, and the functionalized liposomal membranes responded with changes in enzymatic activity, thus serving as an effective nanosensory device for liposome fusion detection. View Full-Text
Scifeed alert for new publicationsNever miss any articles matching your research from any publisher
- Get alerts for new papers matching your research
- Find out the new papers from selected authors
- Updated daily for 49'000+ journals and 6000+ publishers
- Define your Scifeed now
Mukai, M.; Sasaki, Y.; Kikuchi, J.-I. Fusion-Triggered Switching of Enzymatic Activity on an Artificial Cell Membrane. Sensors 2012, 12, 5966-5977.
Mukai M, Sasaki Y, Kikuchi J-I. Fusion-Triggered Switching of Enzymatic Activity on an Artificial Cell Membrane. Sensors. 2012; 12(5):5966-5977.Chicago/Turabian Style
Mukai, Masaru; Sasaki, Yoshihiro; Kikuchi, Jun-ichi. 2012. "Fusion-Triggered Switching of Enzymatic Activity on an Artificial Cell Membrane." Sensors 12, no. 5: 5966-5977.