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Fusion-Triggered Switching of Enzymatic Activity on an Artificial Cell Membrane
Graduate School of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan
Nanotube Research Center, National Institute of Advanced Industrial Science and Technology, Tsukuba Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan
Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda, Tokyo 101-0062, Japan
PRESTO, Japan Science and Technology Agency, 4-1-8 Honcho Kawaguchi, Saitama 332-0012, Japan
* Author to whom correspondence should be addressed.
Received: 14 March 2012; in revised form: 13 April 2012 / Accepted: 3 May 2012 / Published: 9 May 2012
Abstract: A nanosensory membrane device was constructed for detecting liposome fusion through changes in an enzymatic activity. Inspired by a biological signal transduction system, the device design involved functionalized liposomal membranes prepared by self-assembly of the following molecular components: a synthetic peptide lipid and a phospholipid as matrix membrane components, a Schiff’s base of pyridoxal 5’-phosphate with phosphatidylethanolamine as a thermo-responsive artificial receptor, NADH-dependent L-lactate dehydrogenase as a signal amplifier, and Cu2+ ion as a signal mediator between the receptor and enzyme. The enzymatic activity of the membrane device was adjustable by changing the matrix lipid composition, reflecting the thermotropic phase transition behavior of the lipid membranes, which in turn controlled receptor binding affinity toward the enzyme-inhibiting mediator species. When an effective fusogen anionic polymer was added to these cationic liposomes, membrane fusion occurred, and the functionalized liposomal membranes responded with changes in enzymatic activity, thus serving as an effective nanosensory device for liposome fusion detection.
Keywords: liposome; enzyme; liposome fusion; self-assembly; phase transition; molecular device
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Mukai, M.; Sasaki, Y.; Kikuchi, J.-I. Fusion-Triggered Switching of Enzymatic Activity on an Artificial Cell Membrane. Sensors 2012, 12, 5966-5977.
Mukai M, Sasaki Y, Kikuchi J-I. Fusion-Triggered Switching of Enzymatic Activity on an Artificial Cell Membrane. Sensors. 2012; 12(5):5966-5977.
Mukai, Masaru; Sasaki, Yoshihiro; Kikuchi, Jun-ichi. 2012. "Fusion-Triggered Switching of Enzymatic Activity on an Artificial Cell Membrane." Sensors 12, no. 5: 5966-5977.