Sensors 2012, 12(5), 5966-5977; doi:10.3390/s120505966

Fusion-Triggered Switching of Enzymatic Activity on an Artificial Cell Membrane

1,2email, 3,4email and 1,* email
Received: 14 March 2012; in revised form: 13 April 2012 / Accepted: 3 May 2012 / Published: 9 May 2012
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: A nanosensory membrane device was constructed for detecting liposome fusion through changes in an enzymatic activity. Inspired by a biological signal transduction system, the device design involved functionalized liposomal membranes prepared by self-assembly of the following molecular components: a synthetic peptide lipid and a phospholipid as matrix membrane components, a Schiff’s base of pyridoxal 5’-phosphate with phosphatidylethanolamine as a thermo-responsive artificial receptor, NADH-dependent L-lactate dehydrogenase as a signal amplifier, and Cu2+ ion as a signal mediator between the receptor and enzyme. The enzymatic activity of the membrane device was adjustable by changing the matrix lipid composition, reflecting the thermotropic phase transition behavior of the lipid membranes, which in turn controlled receptor binding affinity toward the enzyme-inhibiting mediator species. When an effective fusogen anionic polymer was added to these cationic liposomes, membrane fusion occurred, and the functionalized liposomal membranes responded with changes in enzymatic activity, thus serving as an effective nanosensory device for liposome fusion detection.
Keywords: liposome; enzyme; liposome fusion; self-assembly; phase transition; molecular device
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MDPI and ACS Style

Mukai, M.; Sasaki, Y.; Kikuchi, J.-I. Fusion-Triggered Switching of Enzymatic Activity on an Artificial Cell Membrane. Sensors 2012, 12, 5966-5977.

AMA Style

Mukai M, Sasaki Y, Kikuchi J-I. Fusion-Triggered Switching of Enzymatic Activity on an Artificial Cell Membrane. Sensors. 2012; 12(5):5966-5977.

Chicago/Turabian Style

Mukai, Masaru; Sasaki, Yoshihiro; Kikuchi, Jun-ichi. 2012. "Fusion-Triggered Switching of Enzymatic Activity on an Artificial Cell Membrane." Sensors 12, no. 5: 5966-5977.

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