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Sensors 2012, 12(4), 4311-4323; doi:10.3390/s120404311

Application of Optical Biosensors in Small-Molecule Screening Activities

1,* , 1
1 Discovery Sciences, AstraZeneca R&D Mölndal, 43183 Mölndal, Sweden 2 CVGI iMed, Bioscience, AstraZeneca R&D Mölndal, 43183 Mölndal, Sweden
* Authors to whom correspondence should be addressed.
Received: 22 February 2012 / Revised: 20 March 2012 / Accepted: 23 March 2012 / Published: 28 March 2012
(This article belongs to the Special Issue Sensors and Biosensors Using Label Free Chemistries)
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The last two decades have seen remarkable progress and improvements in optical biosensor systems such that those are currently seen as an important and value-adding component of modern drug screening activities. In particular the introduction of microplate-based biosensor systems holds the promise to match the required throughput without compromising on data quality thus representing a sought-after complement to traditional fluidic systems. This article aims to highlight the application of the two most prominent optical biosensor technologies, namely surface plasmon resonance (SPR) and optical waveguide grating (OWG), in small-molecule screening and will present, review and discuss the advantages and disadvantages of different assay formats on these platforms. A particular focus will be on the specific advantages of the inhibition in solution assay (ISA) format in contrast to traditional direct binding assays (DBA). Furthermore we will discuss different application areas for both fluidic as well as plate-based biosensor systems by considering the individual strength of the platforms.
Keywords: screening; fragment; ligand; biosensor; surface plasmon resonance; optical waveguide grating; drug discovery screening; fragment; ligand; biosensor; surface plasmon resonance; optical waveguide grating; drug discovery
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Geschwindner, S.; Carlsson, J.F.; Knecht, W. Application of Optical Biosensors in Small-Molecule Screening Activities. Sensors 2012, 12, 4311-4323.

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