Next Article in Journal
A Robust Kalman Algorithm to Facilitate Human-Computer Interaction for People with Cerebral Palsy, Using a New Interface Based on Inertial Sensors
Next Article in Special Issue
Application of Optical Biosensors in Small-Molecule Screening Activities
Previous Article in Journal
Size Matters: Problems and Advantages Associated with Highly Miniaturized Sensors
Article Menu

Export Article

Open AccessArticle
Sensors 2012, 12(3), 3037-3048; doi:10.3390/s120303037

A Reusable Impedimetric Aptasensor for Detection of Thrombin Employing a Graphite-Epoxy Composite Electrode

Sensors and Biosensors Group, Department of Chemistry, Universitat Autònoma de Barcelona, Bellaterra 08193, Spain
Author to whom correspondence should be addressed.
Received: 13 January 2012 / Revised: 15 February 2012 / Accepted: 23 February 2012 / Published: 6 March 2012
(This article belongs to the Special Issue Sensors and Biosensors Using Label Free Chemistries)
View Full-Text   |   Download PDF [412 KB, uploaded 21 June 2014]   |  


Here, we report the application of a label-free electrochemical aptasensor based on a graphite-epoxy composite electrode for the detection of thrombin; in this work, aptamers were immobilized onto the electrodes surface using wet physical adsorption. The detection principle is based on the changes of the interfacial properties of the electrode; these were probed in the presence of the reversible redox couple [Fe(CN)6]3−/[Fe(CN)6]4− using impedance measurements. The electrode surface was partially blocked due to formation of aptamer-thrombin complex, resulting in an increase of the interfacial electron-transfer resistance detected by Electrochemical Impedance Spectroscopy (EIS). The aptasensor showed a linear response for thrombin in the range of 7.5 pM to 75 pM and a detection limit of 4.5 pM. The aptasensor was regenerated by breaking the complex formed between the aptamer and thrombin using 2.0 M NaCl solution at 42 °C, showing its operation for different cycles. The interference response caused by main proteins in serum has been characterized.
Keywords: aptamer; thrombin; electrochemical impedance spectroscopy; labeless; adsorption aptamer; thrombin; electrochemical impedance spectroscopy; labeless; adsorption

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Ocaña, C.; Pacios, M.; del Valle, M. A Reusable Impedimetric Aptasensor for Detection of Thrombin Employing a Graphite-Epoxy Composite Electrode. Sensors 2012, 12, 3037-3048.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top