Sensors 2012, 12(3), 3253-3268; doi:10.3390/s120303253
Article

Community Level Physiological Profiles (CLPP), Characterization and Microbial Activity of Soil Amended with Dairy Sewage Sludge

Institute of Agrophysics, Polish Academy of Sciences, Doświadczalna 4, 20-290 Lublin 27, Poland
* Author to whom correspondence should be addressed.
Received: 16 January 2012; in revised form: 23 February 2012 / Accepted: 27 February 2012 / Published: 7 March 2012
(This article belongs to the Section Biosensors)
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Abstract: The aim of the present work was to assess the influence of organic amendment applications compared to mineral fertilization on soil microbial activity and functional diversity. The field experiment was set up on a soil classified as an Eutric Cambisol developed from loess (South-East Poland). Two doses of both dairy sewage sludge (20 Mg·ha−1 and 26 Mg·ha−1) and of mineral fertilizers containing the same amount of nutrients were applied. The same soil without any amendment was used as a control. The soil under undisturbed native vegetation was also included in the study as a representative background sample. The functional diversity (catabolic potential) was assessed using such indices as Average Well Color Development (AWCD), Richness (R) and Shannon–Weaver index (H). These indices were calculated, following the community level physiological profiling (CLPP) using Biolog Eco Plates. Soil dehydrogenase and respiratory activity were also evaluated. The indices were sensitive enough to reveal changes in community level physiological profiles due to treatment effects. It was shown that dairy sewage amended soil was characterized by greater AWCD, R, H and dehydrogenase and respiratory activity as compared to control or mineral fertilized soil. Analysis of variance (ANOVA) and principal component analysis (PCA) were used to depict the differences of the soil bacterial functional diversity between the treatments.
Keywords: microbial diversity; community level physiological profiles; Biolog EcoPlate; dairy sewage sludge; soil

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MDPI and ACS Style

Frąc, M.; Oszust, K.; Lipiec, J. Community Level Physiological Profiles (CLPP), Characterization and Microbial Activity of Soil Amended with Dairy Sewage Sludge. Sensors 2012, 12, 3253-3268.

AMA Style

Frąc M, Oszust K, Lipiec J. Community Level Physiological Profiles (CLPP), Characterization and Microbial Activity of Soil Amended with Dairy Sewage Sludge. Sensors. 2012; 12(3):3253-3268.

Chicago/Turabian Style

Frąc, Magdalena; Oszust, Karolina; Lipiec, Jerzy. 2012. "Community Level Physiological Profiles (CLPP), Characterization and Microbial Activity of Soil Amended with Dairy Sewage Sludge." Sensors 12, no. 3: 3253-3268.

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