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Sensors 2012, 12(3), 2456-2466; doi:10.3390/s120302456

Electrochemical L-Lactic Acid Sensor Based on Immobilized ZnO Nanorods with Lactate Oxidase

Department of Science and Technology, Campus Norrköping, Linköping University, SE-60174, Norrköping, Sweden
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Received: 28 December 2011 / Revised: 29 January 2012 / Accepted: 21 February 2012 / Published: 23 February 2012
(This article belongs to the Section Biosensors)
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Abstract

In this work, fabrication of gold coated glass substrate, growth of ZnO nanorods and potentiometric response of lactic acid are explained. The biosensor was developed by immobilizing the lactate oxidase on the ZnO nanorods in combination with glutaraldehyde as a cross linker for lactate oxidase enzyme. The potentiometric technique was applied for the measuring the output (EMF) response of L-lactic acid biosensor. We noticed that the present biosensor has wide linear detection range of concentration from 1 × 10−4–1 × 100 mM with acceptable sensitivity about 41.33 ± 1.58 mV/decade. In addition, the proposed biosensor showed fast response time less than 10 s, a good selectivity towards L-lactic acid in presence of common interfering substances such as ascorbic acid, urea, glucose, galactose, magnesium ions and calcium ions. The present biosensor based on immobilized ZnO nanorods with lactate oxidase sustained its stability for more than three weeks. View Full-Text
Keywords: zinc oxide nanorods; lactate oxidase enzyme; glutaraldehyde; potentiometric nanostructured biosensor; nanodevices zinc oxide nanorods; lactate oxidase enzyme; glutaraldehyde; potentiometric nanostructured biosensor; nanodevices
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Ibupoto, Z.H.; Shah, S.M.U.A.; Khun, K.; Willander, M. Electrochemical L-Lactic Acid Sensor Based on Immobilized ZnO Nanorods with Lactate Oxidase. Sensors 2012, 12, 2456-2466.

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