Sensors 2012, 12(1), 612-631; doi:10.3390/s120100612
Review

Aptamers and Their Biological Applications

Department of Chemistry, Pohang University of Science and Technology, San31, Hyoja-dong, Pohang, Gyeongbuk 790-784, Korea
* Author to whom correspondence should be addressed.
Received: 29 November 2011; in revised form: 31 December 2011 / Accepted: 4 January 2012 / Published: 9 January 2012
PDF Full-text Download PDF Full-Text [922 KB, uploaded 9 January 2012 13:51 CET]
Abstract: Recently, aptamers have attracted the attention of many scientists, because they not only have all of the advantages of antibodies, but also have unique merits, such as thermal stability, low cost, and unlimited applications. In this review, we present the reasons why aptamers are known as alternatives to antibodies. Furthermore, several types of in vitro selection processes, including nitrocellulose membrane filtration, affinity chromatography, magnetic bead, and capillary electrophoresis-based selection methods, are explained in detail. We also introduce various applications of aptamers for the diagnosis of diseases and detection of small molecules. Numerous analytical techniques, such as electrochemical, colorimetric, optical, and mass-sensitive methods, can be utilized to detect targets, due to convenient modifications and the stability of aptamers. Finally, several medical and analytical applications of aptamers are presented. In summary, aptamers are promising materials for diverse areas, not just as alternatives to antibodies, but as the core components of medical and analytical equipment.
Keywords: aptamer; SELEX; in vitro selection; aptasensor; diagnosis; biosensor

Article Statistics

Load and display the download statistics.

Citations to this Article

Cite This Article

MDPI and ACS Style

Song, K.-M.; Lee, S.; Ban, C. Aptamers and Their Biological Applications. Sensors 2012, 12, 612-631.

AMA Style

Song K-M, Lee S, Ban C. Aptamers and Their Biological Applications. Sensors. 2012; 12(1):612-631.

Chicago/Turabian Style

Song, Kyung-Mi; Lee, Seonghwan; Ban, Changill. 2012. "Aptamers and Their Biological Applications." Sensors 12, no. 1: 612-631.

Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert