Next Article in Journal
Real-Time Gait Cycle Parameter Recognition Using a Wearable Accelerometry System
Previous Article in Journal
Electromechanical Impedance Response of a Cracked Timoshenko Beam
Article Menu

Export Article

Open AccessArticle
Sensors 2011, 11(8), 7302-7313; doi:10.3390/s110807302

An Optical Test Strip for the Detection of Benzoic Acid in Food

1
Faculty of Science, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
2
Faculty of Applied Science, Universiti Teknologi Mara, 26400 Jengka, Pahang, Malaysia
3
Institute of Technology and Advance Material (ITMA), Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
4
Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
5
Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
*
Author to whom correspondence should be addressed.
Received: 10 June 2011 / Revised: 14 July 2011 / Accepted: 20 July 2011 / Published: 25 July 2011
(This article belongs to the Section Chemical Sensors)
View Full-Text   |   Download PDF [271 KB, uploaded 21 June 2014]   |  

Abstract

Fabrication of a test strip for detection of benzoic acid was successfully implemented by immobilizing tyrosinase, phenol and 3-methyl-2-benzothiazolinone hydrazone (MBTH) onto filter paper using polystyrene as polymeric support. The sensing scheme was based on the decreasing intensity of the maroon colour of the test strip when introduced into benzoic acid solution. The test strip was characterized using optical fiber reflectance and has maximum reflectance at 375 nm. It has shown a highly reproducible measurement of benzoic acid with a calculated RSD of 0.47% (n = 10). The detection was optimized at pH 7. A linear response of the biosensor was obtained in 100 to 700 ppm of benzoic acid with a detection limit (LOD) of 73.6 ppm. At 1:1 ratio of benzoic acid to interfering substances, the main interfering substance is boric acid. The kinetic analyses show that, the inhibition of benzoic is competitive inhibitor and the inhibition constant (Ki) is 52.9 ppm. The activity of immobilized tyrosinase, phenol, and MBTH in the test strip was fairly sustained during 20 days when stored at 3 °C. The developed test strip was used for detection of benzoic acid in food samples and was observed to have comparable results to the HPLC method, hence the developed test strip can be used as an alternative to HPLC in detecting benzoic acid in food products. View Full-Text
Keywords: optical biosensor; test strip; tyrosinase; benzoic acid optical biosensor; test strip; tyrosinase; benzoic acid
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Hamzah, H.H.; Yusof, N.A.; Salleh, A.B.; Bakar, F.A. An Optical Test Strip for the Detection of Benzoic Acid in Food. Sensors 2011, 11, 7302-7313.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top