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Cell Docking, Movement and Cell-Cell Interactions of Heterogeneous Cell Suspensions in a Cell Manipulation Microdevice
Institute of Applied Mechanics, National Taiwan University, 1 Sec. 4 Roosevelt Road, Taipei 10617, Taiwan
Bioresources Collection and Research Center, Food Industry Research and Development Institute, 331 Shih-Pin Road, Hsinchu 300, Taiwan
* Author to whom correspondence should be addressed.
Received: 2 September 2011; in revised form: 22 September 2011 / Accepted: 29 September 2011 / Published: 12 October 2011
Abstract: This study demonstrates a novel cell manipulation microdevice for cell docking, culturing, cell-cell contact and interaction by microfluidic manipulation of heterogeneous cell suspensions. Heterogeneous cell suspensions include disparate blood cells of natural killer cells and leukemia cancer cells for immune cell transplantation therapy. However, NK cell alloreactivity from different healthy donors present various recovery response levels. Little is still known about the interactions and cytotoxicity effects between donor NK cells and recipient cancer cells. The cell-based micro device first showed the capability of cell docking, movement, contact and cell-cell interaction with respect to cell cytotoxicity of NK cells against cancer cells. With various flow tests for live cell loading, flow rates of 10 μL/h were chosen for injection in the central and side flows such that both types of suspension cells could be gently docked at the gap structure in a reaction zone. The trapping number of particles and cells was linearly proportional to the gap length. Finally, the cytotoxicity of around 40% was found to be similar in the case of dilute cells and a large cell population. As a result, the cell manipulation microdevice has been validated for live suspensions of natural killer and cancer cells, and exhibited the capability to measure the cytotoxicity of dilute cell suspensions.
Keywords: Natural Killer cells; microfluidics; MEMS; cell-cell interaction; cytotoxicity; microchannel
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Lai, F.-L.; Wang, Y.-H.; Chung, Y.-W.; Hwang, S.-M.; Huang, L.-S. Cell Docking, Movement and Cell-Cell Interactions of Heterogeneous Cell Suspensions in a Cell Manipulation Microdevice. Sensors 2011, 11, 9613-9627.
Lai F-L, Wang Y-H, Chung Y-W, Hwang S-M, Huang L-S. Cell Docking, Movement and Cell-Cell Interactions of Heterogeneous Cell Suspensions in a Cell Manipulation Microdevice. Sensors. 2011; 11(10):9613-9627.
Lai, Fei-Lung; Wang, Yu-Hung; Chung, Yu-Wei; Hwang, Shiaw-Min; Huang, Long-Sun. 2011. "Cell Docking, Movement and Cell-Cell Interactions of Heterogeneous Cell Suspensions in a Cell Manipulation Microdevice." Sensors 11, no. 10: 9613-9627.