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A Conductometric Indium Oxide Semiconducting Nanoparticle Enzymatic Biosensor Array
Department of Mechanical Engineering, University of Minnesota, 111 Church St. S.E., Minneapolis, MN 55455, USA
Department of Mechanical Engineering, Ohio Northern University, 525 South Main St., Ada, OH 45810, USA
* Author to whom correspondence should be addressed.
Received: 4 August 2011; in revised form: 4 September 2011 / Accepted: 23 September 2011 / Published: 28 September 2011
Abstract: We report a conductometric nanoparticle biosensor array to address the significant variation of electrical property in nanomaterial biosensors due to the random network nature of nanoparticle thin-film. Indium oxide and silica nanoparticles (SNP) are assembled selectively on the multi-site channel area of the resistors using layer-by-layer self-assembly. To demonstrate enzymatic biosensing capability, glucose oxidase is immobilized on the SNP layer for glucose detection. The packaged sensor chip onto a ceramic pin grid array is tested using syringe pump driven feed and multi-channel I–V measurement system. It is successfully demonstrated that glucose is detected in many different sensing sites within a chip, leading to concentration dependent currents. The sensitivity has been found to be dependent on the channel length of the resistor, 4–12 nA/mM for channel lengths of 5–20 µm, while the apparent Michaelis-Menten constant is 20 mM. By using sensor array, analytical data could be obtained with a single step of sample solution feeding. This work sheds light on the applicability of the developed nanoparticle microsensor array to multi-analyte sensors, novel bioassay platforms, and sensing components in a lab-on-a-chip.
Keywords: biosensor array; nanoparticle; conductometric sensor; microsensor array; glucose sensor
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MDPI and ACS Style
Lee, D.; Ondrake, J.; Cui, T. A Conductometric Indium Oxide Semiconducting Nanoparticle Enzymatic Biosensor Array. Sensors 2011, 11, 9300-9312.
Lee D, Ondrake J, Cui T. A Conductometric Indium Oxide Semiconducting Nanoparticle Enzymatic Biosensor Array. Sensors. 2011; 11(10):9300-9312.
Lee, Dongjin; Ondrake, Janet; Cui, Tianhong. 2011. "A Conductometric Indium Oxide Semiconducting Nanoparticle Enzymatic Biosensor Array." Sensors 11, no. 10: 9300-9312.