Sensors 2011, 11(10), 9300-9312; doi:10.3390/s111009300
Article

A Conductometric Indium Oxide Semiconducting Nanoparticle Enzymatic Biosensor Array

1 Department of Mechanical Engineering, University of Minnesota, 111 Church St. S.E., Minneapolis, MN 55455, USA 2 Department of Mechanical Engineering, Ohio Northern University, 525 South Main St., Ada, OH 45810, USA
* Author to whom correspondence should be addressed.
Received: 4 August 2011; in revised form: 4 September 2011 / Accepted: 23 September 2011 / Published: 28 September 2011
(This article belongs to the Special Issue Nano-Biosensors)
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Abstract: We report a conductometric nanoparticle biosensor array to address the significant variation of electrical property in nanomaterial biosensors due to the random network nature of nanoparticle thin-film. Indium oxide and silica nanoparticles (SNP) are assembled selectively on the multi-site channel area of the resistors using layer-by-layer self-assembly. To demonstrate enzymatic biosensing capability, glucose oxidase is immobilized on the SNP layer for glucose detection. The packaged sensor chip onto a ceramic pin grid array is tested using syringe pump driven feed and multi-channel I–V measurement system. It is successfully demonstrated that glucose is detected in many different sensing sites within a chip, leading to concentration dependent currents. The sensitivity has been found to be dependent on the channel length of the resistor, 4–12 nA/mM for channel lengths of 5–20 µm, while the apparent Michaelis-Menten constant is 20 mM. By using sensor array, analytical data could be obtained with a single step of sample solution feeding. This work sheds light on the applicability of the developed nanoparticle microsensor array to multi-analyte sensors, novel bioassay platforms, and sensing components in a lab-on-a-chip.
Keywords: biosensor array; nanoparticle; conductometric sensor; microsensor array; glucose sensor

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MDPI and ACS Style

Lee, D.; Ondrake, J.; Cui, T. A Conductometric Indium Oxide Semiconducting Nanoparticle Enzymatic Biosensor Array. Sensors 2011, 11, 9300-9312.

AMA Style

Lee D, Ondrake J, Cui T. A Conductometric Indium Oxide Semiconducting Nanoparticle Enzymatic Biosensor Array. Sensors. 2011; 11(10):9300-9312.

Chicago/Turabian Style

Lee, Dongjin; Ondrake, Janet; Cui, Tianhong. 2011. "A Conductometric Indium Oxide Semiconducting Nanoparticle Enzymatic Biosensor Array." Sensors 11, no. 10: 9300-9312.

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