Next Article in Journal
Hall-Effect Based Semi-Fast AC On-Board Charging Equipment for Electric Vehicles
Next Article in Special Issue
A Nanostructured Piezoelectric Immunosensor for Detection of Human Cardiac Troponin T
Previous Article in Journal
The Potential for Harvesting Energy from the Movement of Trees
Previous Article in Special Issue
Biofunctionalized Zinc Oxide Field Effect Transistors for Selective Sensing of Riboflavin with Current Modulation
Sensors 2011, 11(10), 9300-9312; doi:10.3390/s111009300

A Conductometric Indium Oxide Semiconducting Nanoparticle Enzymatic Biosensor Array

1 Department of Mechanical Engineering, University of Minnesota, 111 Church St. S.E., Minneapolis, MN 55455, USA 2 Department of Mechanical Engineering, Ohio Northern University, 525 South Main St., Ada, OH 45810, USA
* Author to whom correspondence should be addressed.
Received: 4 August 2011 / Revised: 4 September 2011 / Accepted: 23 September 2011 / Published: 28 September 2011
(This article belongs to the Special Issue Nano-Biosensors)
View Full-Text   |   Download PDF [1713 KB, uploaded 21 June 2014]   |   Browse Figures


We report a conductometric nanoparticle biosensor array to address the significant variation of electrical property in nanomaterial biosensors due to the random network nature of nanoparticle thin-film. Indium oxide and silica nanoparticles (SNP) are assembled selectively on the multi-site channel area of the resistors using layer-by-layer self-assembly. To demonstrate enzymatic biosensing capability, glucose oxidase is immobilized on the SNP layer for glucose detection. The packaged sensor chip onto a ceramic pin grid array is tested using syringe pump driven feed and multi-channel I–V measurement system. It is successfully demonstrated that glucose is detected in many different sensing sites within a chip, leading to concentration dependent currents. The sensitivity has been found to be dependent on the channel length of the resistor, 4–12 nA/mM for channel lengths of 5–20 µm, while the apparent Michaelis-Menten constant is 20 mM. By using sensor array, analytical data could be obtained with a single step of sample solution feeding. This work sheds light on the applicability of the developed nanoparticle microsensor array to multi-analyte sensors, novel bioassay platforms, and sensing components in a lab-on-a-chip.
Keywords: biosensor array; nanoparticle; conductometric sensor; microsensor array; glucose sensor biosensor array; nanoparticle; conductometric sensor; microsensor array; glucose sensor
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote |
MDPI and ACS Style

Lee, D.; Ondrake, J.; Cui, T. A Conductometric Indium Oxide Semiconducting Nanoparticle Enzymatic Biosensor Array. Sensors 2011, 11, 9300-9312.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here


[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert