Sensors 2011, 11(7), 6645-6655; doi:10.3390/s110706645
Communication

Biofunctionalized Zinc Oxide Field Effect Transistors for Selective Sensing of Riboflavin with Current Modulation

Received: 1 May 2011; in revised form: 1 June 2011 / Accepted: 20 June 2011 / Published: 27 June 2011
(This article belongs to the Special Issue Nano-Biosensors)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Zinc oxide field effect transistors (ZnO-FET), covalently functionalized with single stranded DNA aptamers, provide a highly selective platform for label-free small molecule sensing. The nanostructured surface morphology of ZnO provides high sensitivity and room temperature deposition allows for a wide array of substrate types. Herein we demonstrate the selective detection of riboflavin down to the pM level in aqueous solution using the negative electrical current response of the ZnO-FET by covalently attaching a riboflavin binding aptamer to the surface. The response of the biofunctionalized ZnO-FET was tuned by attaching a redox tag (ferrocene) to the 3’ terminus of the aptamer, resulting in positive current modulation upon exposure to riboflavin down to pM levels.
Keywords: aptamer; biomolecular detection; ZnO-FET; sensor; riboflavin; selectivity; label-free; biofunctionalization
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MDPI and ACS Style

Hagen, J.A.; Kim, S.N.; Bayraktaroglu, B.; Leedy, K.; Chávez, J.L.; Kelley-Loughnane, N.; Naik, R.R.; Stone, M.O. Biofunctionalized Zinc Oxide Field Effect Transistors for Selective Sensing of Riboflavin with Current Modulation. Sensors 2011, 11, 6645-6655.

AMA Style

Hagen JA, Kim SN, Bayraktaroglu B, Leedy K, Chávez JL, Kelley-Loughnane N, Naik RR, Stone MO. Biofunctionalized Zinc Oxide Field Effect Transistors for Selective Sensing of Riboflavin with Current Modulation. Sensors. 2011; 11(7):6645-6655.

Chicago/Turabian Style

Hagen, Joshua A.; Kim, Sang N.; Bayraktaroglu, Burhan; Leedy, Kevin; Chávez, Jorge L.; Kelley-Loughnane, Nancy; Naik, Rajesh R.; Stone, Morley O. 2011. "Biofunctionalized Zinc Oxide Field Effect Transistors for Selective Sensing of Riboflavin with Current Modulation." Sensors 11, no. 7: 6645-6655.

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