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A Nanostructured Piezoelectric Immunosensor for Detection of Human Cardiac Troponin T
Laboratório de Engenharia Biomédica, Universidade Federal de Pernambuco, Av. Prof. Moraes Rego, 1235, Recife, Pernambuco 50670-901, Brazil
Instituto de Química, Universidade Estadual de Campinas, Campinas 04118-080, SP, Brazil
* Author to whom correspondence should be addressed.
Received: 10 October 2011; in revised form: 2 November 2011 / Accepted: 9 November 2011 / Published: 16 November 2011
Abstract: A piezoelectric immunosensor based on gold nanoparticles (AuNPs) co-immobilized on a dithiol-modified surface is proposed for detection of human cardiac troponin T (TnT). Anti-human troponin T (anti-TnT) antibodies were covalently immobilized on the nanostructured electrode surface by thiol-aldehyde linkages. In a homogeneous bulk solution, TnT was captured by anti-TnT immobilized on the QCM electrode. Cyclic voltammetry studies were used to characterize the AuNPs layer on the electrode surface and the anti-TnT immobilization steps. The QCM-flow immunosensor exhibited good reliability, measuring concentrations of TnT from 0.003 to 0.5 ng mL−1 in human serum with high linearity (r = 0.989; p < 0.01). The immunosensor exhibited a 7% coefficient of variation and 0.0015 ng mL−1 limit of detection, indicating a high reproducibility and sensitivity. The proposed QCM nanostructured immunosensor is easy to use and has promising potential in the diagnosis of acute myocardial infarction due to its speed and high sensitivity.
Keywords: piezoelectric; immunosensor; gold nanoparticle; cardiac troponin T
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Fonseca, R.A.S.; Ramos-Jesus, J.; Kubota, L.T.; Dutra, R.F. A Nanostructured Piezoelectric Immunosensor for Detection of Human Cardiac Troponin T. Sensors 2011, 11, 10785-10797.
Fonseca RAS, Ramos-Jesus J, Kubota LT, Dutra RF. A Nanostructured Piezoelectric Immunosensor for Detection of Human Cardiac Troponin T. Sensors. 2011; 11(11):10785-10797.
Fonseca, Rosana A. S.; Ramos-Jesus, Joilson; Kubota, Lauro T.; Dutra, Rosa F. 2011. "A Nanostructured Piezoelectric Immunosensor for Detection of Human Cardiac Troponin T." Sensors 11, no. 11: 10785-10797.