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Sensors 2010, 10(7), 6969-6979; doi:10.3390/s100706969

Article

Exploiting the Autofluorescent Properties of Photosynthetic Pigments for Analysis of Pigmentation and Morphology in Live Fremyella diplosiphon Cells

Juliana R. Bordowitz ^1,2,3  and Beronda L. Montgomery ^1,2,4,*

¹ Plant Research Laboratory, Department of Energy, Michigan State University, 106 Plant Biology Building, East Lansing, MI 48824-1312, USA ² Cell and Molecular Biology Program, Michigan State University, 2240A Biomedical and Physical Science Building, East Lansing, MI 48824-4320, USA ³ Division of Biological Sciences, University of California-San Diego, La Jolla, CA 92093-0116, USA ⁴ Department of Biochemistry and Molecular Biology, Michigan State University, 210 Biochemistry, East Lansing, MI 48824-1319, USA

* Author to whom correspondence should be addressed.

Received: 7 June 2010; in revised form: 12 July 2010 / Accepted: 16 July 2010 / Published: 19 July 2010

(This article belongs to the Special Issue Photodetectors and Imaging Technologies)

Download PDF Full-Text [343 KB, uploaded 19 July 2010 13:39 CEST]

Abstract: Fremyella diplosiphon is a freshwater, filamentous cyanobacterium that exhibits light-dependent regulation of photosynthetic pigment accumulation and cellular and filament morphologies in a well-known process known as complementary chromatic adaptation (CCA). One of the techniques used to investigate the molecular bases of distinct aspects of CCA is confocal laser scanning microscopy (CLSM). CLSM capitalizes on the autofluorescent properties of cyanobacterial phycobiliproteins and chlorophyll a. We employed CLSM to perform spectral scanning analyses of F. diplosiphon strains grown under distinct light conditions. We report optimized utilization of CLSM to elucidate the molecular basis of the photoregulation of pigment accumulation and morphological responses in F. diplosiphon.

Keywords: autofluorescence; confocal laser scanning microscopy; cyanobacteria; fluorescence imaging; light; microscopy; morphology; phycobiliproteins

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