Next Article in Journal
L Band Brightness Temperature Observations over a Corn Canopy during the Entire Growth Cycle
Next Article in Special Issue
Micro Cantilever Movement Detection with an Amorphous Silicon Array of Position Sensitive Detectors
Previous Article in Journal
Wireless Sensor Networks for Oceanographic Monitoring: A Systematic Review
Previous Article in Special Issue
Semiconducting Polymer Photodetectors with Electron and Hole Blocking Layers: High Detectivity in the Near-Infrared
Article Menu

Export Article

Open AccessArticle
Sensors 2010, 10(7), 6969-6979; doi:10.3390/s100706969

Exploiting the Autofluorescent Properties of Photosynthetic Pigments for Analysis of Pigmentation and Morphology in Live Fremyella diplosiphon Cells

1
Plant Research Laboratory, Department of Energy, Michigan State University, 106 Plant Biology Building, East Lansing, MI 48824-1312, USA
2
Cell and Molecular Biology Program, Michigan State University, 2240A Biomedical and Physical Science Building, East Lansing, MI 48824-4320, USA
3
Division of Biological Sciences, University of California-San Diego, La Jolla, CA 92093-0116, USA
4
Department of Biochemistry and Molecular Biology, Michigan State University, 210 Biochemistry, East Lansing, MI 48824-1319, USA
*
Author to whom correspondence should be addressed.
Received: 7 June 2010 / Revised: 12 July 2010 / Accepted: 16 July 2010 / Published: 19 July 2010
(This article belongs to the Special Issue Photodetectors and Imaging Technologies)
View Full-Text   |   Download PDF [343 KB, uploaded 21 June 2014]   |  

Abstract

Fremyella diplosiphon is a freshwater, filamentous cyanobacterium that exhibits light-dependent regulation of photosynthetic pigment accumulation and cellular and filament morphologies in a well-known process known as complementary chromatic adaptation (CCA). One of the techniques used to investigate the molecular bases of distinct aspects of CCA is confocal laser scanning microscopy (CLSM). CLSM capitalizes on the autofluorescent properties of cyanobacterial phycobiliproteins and chlorophyll a. We employed CLSM to perform spectral scanning analyses of F. diplosiphon strains grown under distinct light conditions. We report optimized utilization of CLSM to elucidate the molecular basis of the photoregulation of pigment accumulation and morphological responses in F. diplosiphon.
Keywords: autofluorescence; confocal laser scanning microscopy; cyanobacteria; fluorescence imaging; light; microscopy; morphology; phycobiliproteins autofluorescence; confocal laser scanning microscopy; cyanobacteria; fluorescence imaging; light; microscopy; morphology; phycobiliproteins
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Bordowitz, J.R.; Montgomery, B.L. Exploiting the Autofluorescent Properties of Photosynthetic Pigments for Analysis of Pigmentation and Morphology in Live Fremyella diplosiphon Cells. Sensors 2010, 10, 6969-6979.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top