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Sensors 2010, 10(7), 6969-6979; doi:10.3390/s100706969
Article

Exploiting the Autofluorescent Properties of Photosynthetic Pigments for Analysis of Pigmentation and Morphology in Live Fremyella diplosiphon Cells

1,2,3
 and
1,2,4,*
Received: 7 June 2010 / Revised: 12 July 2010 / Accepted: 16 July 2010 / Published: 19 July 2010
(This article belongs to the Special Issue Photodetectors and Imaging Technologies)
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Abstract

Fremyella diplosiphon is a freshwater, filamentous cyanobacterium that exhibits light-dependent regulation of photosynthetic pigment accumulation and cellular and filament morphologies in a well-known process known as complementary chromatic adaptation (CCA). One of the techniques used to investigate the molecular bases of distinct aspects of CCA is confocal laser scanning microscopy (CLSM). CLSM capitalizes on the autofluorescent properties of cyanobacterial phycobiliproteins and chlorophyll a. We employed CLSM to perform spectral scanning analyses of F. diplosiphon strains grown under distinct light conditions. We report optimized utilization of CLSM to elucidate the molecular basis of the photoregulation of pigment accumulation and morphological responses in F. diplosiphon.
Keywords: autofluorescence; confocal laser scanning microscopy; cyanobacteria; fluorescence imaging; light; microscopy; morphology; phycobiliproteins autofluorescence; confocal laser scanning microscopy; cyanobacteria; fluorescence imaging; light; microscopy; morphology; phycobiliproteins
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Bordowitz, J.R.; Montgomery, B.L. Exploiting the Autofluorescent Properties of Photosynthetic Pigments for Analysis of Pigmentation and Morphology in Live Fremyella diplosiphon Cells. Sensors 2010, 10, 6969-6979.

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