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Sensors 2010, 10(6), 6172-6194; doi:10.3390/s100606172

SirT1—A Sensor for Monitoring Self-Renewal and Aging Process in Retinal Stem Cells

2,5,6,*  and 3,4,6,*
Received: 8 January 2010 / Revised: 28 February 2010 / Accepted: 3 May 2010 / Published: 21 June 2010
(This article belongs to the Special Issue Delft Workshop 2008-2009—Sensors and Imagers: a VLSI Perspective)
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Retinal stem cells bear potency of proliferation, self-renewal, and differentiation into many retinal cells. Utilizing appropriate sensors one can effectively detect the self-renewal and aging process abilities. Silencing information regulator (SirT1), a member of the sirtuin family, is a NAD-dependent histone deacetylase and an essential mediator for longevity in normal cells by calorie restriction. We firstly investigate the SirT1 mRNA expression in retinal stem cells from rats and 19 human eyes of different ages. Results revealed that SirT1 expression was significantly decreased in in vivo aged eyes, associated with poor self-renewal abilities. Additionally, SirT1 mRNA levels were dose-dependently increased in resveratrol- treated retinal stem cells. The expression of SirT1 on oxidative stress-induced damage was significantly decreased, negatively correlated with the level of intracellular reactive oxygen species production. Treatment with resveratrol could effectively further reduce oxidative stress induced by H2O2 treatment in retinal stem cells. Importantly, the anti-oxidant effects of resveratrol in H2O2-treated retinal stem cells were significantly abolished by knockdown of SirT1 expression (sh-SirT1). SirT1 expression provides a feasible sensor in assessing self-renewal and aging process in retinal stem cells. Resveratrol can prevent reactive oxygen species-induced damages via increased retinal SirT1 expression.
Keywords: retinal stem cells; aging; SirT1; resveratrol retinal stem cells; aging; SirT1; resveratrol
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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Peng, C.-H.; Chang, Y.-L.; Kao, C.-L.; Tseng, L.-M.; Wu, C.-C.; Chen, Y.-C.; Tsai, C.-Y.; Woung, L.-C.; Liu, J.-H.; Chiou, S.-H.; Chen, S.-J. SirT1—A Sensor for Monitoring Self-Renewal and Aging Process in Retinal Stem Cells. Sensors 2010, 10, 6172-6194.

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