Next Article in Journal
Ca2+-Regulated Photoproteins: Effective Immunoassay Reporters
Previous Article in Journal
Approximate Nearest Neighbor Search by Residual Vector Quantization
Article Menu

Export Article

Open AccessArticle
Sensors 2010, 10(12), 11274-11286; doi:10.3390/s101211274

A High-Throughput Enzyme Assay for Organophosphate Residues in Milk

Biosensor Laboratory, Department of Chemistry, BITS, Pilani-K K Birla Goa Campus, Goa, 403726, India
*
Author to whom correspondence should be addressed.
Received: 17 September 2010 / Revised: 15 November 2010 / Accepted: 7 December 2010 / Published: 9 December 2010
(This article belongs to the Section Biosensors)
View Full-Text   |   Download PDF [306 KB, uploaded 21 June 2014]   |  

Abstract

A rapid, high-sensitivity, chemiluminescence (CL) enzyme assay for the determination of organophosphate (OP) residues in milk is presented. The assay for quantification of OP residues in milk is based on the inhibition of enzyme butyrylcholinesterase (BuChE). BuChE was stabilized and preloaded in 384 well plates at 30 °C. The assay permits rapid determination of OPs in milk within 12 min including an incubation step. The enzyme assay was tested for individual and mixtures of OPs such as methyl paraoxon (MPOx), methyl parathion (MP) and malathion (MT) in milk to evaluate their synergistic effect on BuChE inhibition. Good linearity was obtained in the range 0.005–50 µg·L−1 for MPOx and 0.5–1,000 µg·L−1 for MP as well as MT in milk. Mean recovery of 93.2%–98.6% was obtained for MPOx spiked milk samples with 0.99%–1.67% reproducibility (RSD). The proposed method facilitated rapid screening of milk samples in 384 well plate formats with further miniaturization presented in 1,536 well plates.
Keywords: stabilized butyrylcholinesterase; organophosphate; milk; high throughput; chemiluminescence; bioassay stabilized butyrylcholinesterase; organophosphate; milk; high throughput; chemiluminescence; bioassay
Figures

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Mishra, R.K.; Deshpande, K.; Bhand, S. A High-Throughput Enzyme Assay for Organophosphate Residues in Milk. Sensors 2010, 10, 11274-11286.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top