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Sensors 2010, 10(1), 876-889; doi:10.3390/s100100876

Fluorescence-based Sensing of 2,4,6-Trinitrotoluene (TNT) Using a Multi-channeled Poly(methyl methacrylate) (PMMA) Microimmunosensor

* ,
Center for Bio/Molecular Science and Engineering (Code 6920), US Naval Research Laboratory, 4555 Overlook Ave. SW, Washington, DC 20375, USA
* Author to whom correspondence should be addressed.
Received: 31 December 2009 / Revised: 15 January 2010 / Accepted: 19 January 2010 / Published: 22 January 2010
(This article belongs to the Special Issue Fluorescent Chemosensors)
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Fluorescence immunoassays employing monoclonal antibodies directed against the explosive 2,4,6-trinitrotoluene (TNT) were conducted in a multi-channel microimmunosensor. The multi-channel microimmunosensor was prepared in poly (methyl methacrylate) (PMMA) via hot embossing from a brass molding tool. The multi-channeled microfluidic device was sol-gel coated to generate a siloxane surface that provided a scaffold for antibody immobilization. AlexaFluor-cadaverine-trinitrobenzene (AlexaFluor-Cad-TNB) was used as the reporter molecule in a displacement immunoassay. The limit of detection was 1-10 ng/mL (ppb) with a linear dynamic range that covered three orders of magnitude. In addition, antibody crossreactivity was investigated using hexahydro-1,3,5-triazine (RDX), HMX, 2,4-dinitrotoluene (DNT), 4-nitrotoluene (4-NT) and 2-amino-4,6-DNT.
Keywords: TNT; immunosensor; fluorescence; PMMA; microchannel; antibody TNT; immunosensor; fluorescence; PMMA; microchannel; antibody
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Charles, P.T.; Adams, A.A.; Howell, P.B.; Trammell, S.A.; Deschamps, J.R.; Kusterbeck, A.W. Fluorescence-based Sensing of 2,4,6-Trinitrotoluene (TNT) Using a Multi-channeled Poly(methyl methacrylate) (PMMA) Microimmunosensor. Sensors 2010, 10, 876-889.

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