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Sensors 2010, 10(1), 876-889; doi:10.3390/s100100876
Article

Fluorescence-based Sensing of 2,4,6-Trinitrotoluene (TNT) Using a Multi-channeled Poly(methyl methacrylate) (PMMA) Microimmunosensor

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Center for Bio/Molecular Science and Engineering (Code 6920), US Naval Research Laboratory, 4555 Overlook Ave. SW, Washington, DC 20375, USA
* Author to whom correspondence should be addressed.
Received: 31 December 2009 / Revised: 15 January 2010 / Accepted: 19 January 2010 / Published: 22 January 2010
(This article belongs to the Special Issue Fluorescent Chemosensors)
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Abstract

Fluorescence immunoassays employing monoclonal antibodies directed against the explosive 2,4,6-trinitrotoluene (TNT) were conducted in a multi-channel microimmunosensor. The multi-channel microimmunosensor was prepared in poly (methyl methacrylate) (PMMA) via hot embossing from a brass molding tool. The multi-channeled microfluidic device was sol-gel coated to generate a siloxane surface that provided a scaffold for antibody immobilization. AlexaFluor-cadaverine-trinitrobenzene (AlexaFluor-Cad-TNB) was used as the reporter molecule in a displacement immunoassay. The limit of detection was 1-10 ng/mL (ppb) with a linear dynamic range that covered three orders of magnitude. In addition, antibody crossreactivity was investigated using hexahydro-1,3,5-triazine (RDX), HMX, 2,4-dinitrotoluene (DNT), 4-nitrotoluene (4-NT) and 2-amino-4,6-DNT.
Keywords: TNT; immunosensor; fluorescence; PMMA; microchannel; antibody TNT; immunosensor; fluorescence; PMMA; microchannel; antibody
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Charles, P.T.; Adams, A.A.; Howell, P.B.; Trammell, S.A.; Deschamps, J.R.; Kusterbeck, A.W. Fluorescence-based Sensing of 2,4,6-Trinitrotoluene (TNT) Using a Multi-channeled Poly(methyl methacrylate) (PMMA) Microimmunosensor. Sensors 2010, 10, 876-889.

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