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Sensors 2010, 10(2), 1326-1337; doi:10.3390/s100201326

A Lanthanide-Based Chemosensor for Bioavailable Fe3+ Using a Fluorescent Siderophore: An Assay Displacement Approach

1
Department of Marine Science, University of Southern Mississippi, 1020 Balch Blvd. Stennis Space Center, MS 39529, USA
2
Department of Chemistry and Biochemistry, University of Southern Mississippi, 118 College Drive, Hattiesburg, MS 39406, USA
*
Author to whom correspondence should be addressed.
Received: 24 December 2009 / Revised: 28 January 2010 / Accepted: 5 February 2010 / Published: 11 February 2010
(This article belongs to the Special Issue Fluorescent Chemosensors)
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Abstract

The measurement of trace analytes in aqueous systems has become increasingly important for understanding ocean primary productivity. In oceanography, iron (Fe) is a key element in regulating ocean productivity, microplankton assemblages and has been identified as a causative element in the development of some harmful algal blooms.The chemosenor developed in this study is based on an indicator displacement approach that utilizes time-resolved fluorescence and fluorescence resonance energy transfer as the sensing mechanism to achieve detection of Fe3+ ions as low as 5 nM. This novel approach holds promise for the development of photoactive chemosensors for ocean deployment.
Keywords: lanthanide; desferrioxamine; siderophore; iron; chemosensor; indicator displacement assay lanthanide; desferrioxamine; siderophore; iron; chemosensor; indicator displacement assay
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Orcutt, K.M.; Jones, W.S.; McDonald, A.; Schrock, D.; Wallace, K.J. A Lanthanide-Based Chemosensor for Bioavailable Fe3+ Using a Fluorescent Siderophore: An Assay Displacement Approach. Sensors 2010, 10, 1326-1337.

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